On the residual water content of dried but viable cells

Summary

We have examined the residual water in dried cysts of the brine shrimp,Artemia salina, by gas-bombardment techniques at reduced pressures and temperatures. This treatment reduced, but did not remove all the residual water, the lower limit being about 0.0069 gH2O/g dried weight. The significance of such small amounts of water to cell hydration is assessed. The treatment did not appreciably reduce the viability of this cyst population.

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References

  1. 2

    H.T. Meryman, Ann. N.Y. Acad. Sci.85, 729 (1960).

    PubMed  Google Scholar 

  2. 3

    R. Neihof, J.K. Thompson and V.R. Deitz, Nature, Lond.216, 1304 (1967).

    Google Scholar 

  3. 4

    D. Greiff and W.A. Rightsel, Appl. Microbiol.17, 830 (1969).

    PubMed  Google Scholar 

  4. 5

    T.W. Rowe, in: Current Trends in Cryobiology, p. 61. Ed. A.U. Smith, Plenum Press, New York 1970.

    Google Scholar 

  5. 6

    J. Levitt, Responses of Plants to Environmental Stresses. Academic Press, New York 1972.

    Google Scholar 

  6. 7

    A.S. Sussman and H.A. Douthit, A. Rev. Pl. Physiol.24, 311 (1973).

    Article  Google Scholar 

  7. 8

    D. Keilin, Proc. R. Soc., Lond. B150, 149 (1959).

    Google Scholar 

  8. 9

    J.H. Crowe and J.S. Clegg, (Ed.), Anhydrobiosis. Dowden, Hutchinson and Ross, Inc., Stroudsburg, Pennsylvania 1973.

    Google Scholar 

  9. 10

    N. Grossowitz, S. Hestrin and A. Keynan (Ed.) Cryptobiotic Stages in Biological Systems Elsevier, New York 1961.

    Google Scholar 

  10. 11

    F.J. Finamore and J.S. Clegg, In: The Cell-Cycle: Gene-Enzyme Interactions, p. 249. Ed. G.M. Padilla, G.L. Whitson and I.L. Cameron Academic Press, New York 1969.

    Google Scholar 

  11. 12

    J.S. Clegg, Trans Am. microsc. Soc.93, 481 (1974).

    Google Scholar 

  12. 13

    T. Iwasaki, in: Inst. Internat. Froid. Commission C-1, 79–88. Sapporo, Japan 1973–1975.

  13. 14

    J.S. Clegg, J. exp. Biol.61, 291 (1974).

    PubMed  Google Scholar 

  14. 15

    S. Brunauer, Physical Adsorption of Gases & Vapors. Princeton University Press, New Jersey 1943.

    Google Scholar 

  15. 16

    A.C. Zettlemoyer, E.D. Schweitzer and W.C. Walker, J. Am. Leath. Chem. Ass.41, 253 (1946).

    Google Scholar 

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Supported in part by grant PCM-24037 from the US National Science Foundation.

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Clegg, J.S., Zettlemoyer, A.C. & Hsing, H.H. On the residual water content of dried but viable cells. Experientia 34, 734–735 (1978). https://doi.org/10.1007/BF01947290

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Keywords

  • Hydration
  • Viable Cell
  • Lower Limit
  • Residual Water
  • Brine Shrimp