Summary
We have examined the residual water in dried cysts of the brine shrimp,Artemia salina, by gas-bombardment techniques at reduced pressures and temperatures. This treatment reduced, but did not remove all the residual water, the lower limit being about 0.0069 gH2O/g dried weight. The significance of such small amounts of water to cell hydration is assessed. The treatment did not appreciably reduce the viability of this cyst population.
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References
H.T. Meryman, Ann. N.Y. Acad. Sci.85, 729 (1960).
R. Neihof, J.K. Thompson and V.R. Deitz, Nature, Lond.216, 1304 (1967).
D. Greiff and W.A. Rightsel, Appl. Microbiol.17, 830 (1969).
T.W. Rowe, in: Current Trends in Cryobiology, p. 61. Ed. A.U. Smith, Plenum Press, New York 1970.
J. Levitt, Responses of Plants to Environmental Stresses. Academic Press, New York 1972.
A.S. Sussman and H.A. Douthit, A. Rev. Pl. Physiol.24, 311 (1973).
D. Keilin, Proc. R. Soc., Lond. B150, 149 (1959).
J.H. Crowe and J.S. Clegg, (Ed.), Anhydrobiosis. Dowden, Hutchinson and Ross, Inc., Stroudsburg, Pennsylvania 1973.
N. Grossowitz, S. Hestrin and A. Keynan (Ed.) Cryptobiotic Stages in Biological Systems Elsevier, New York 1961.
F.J. Finamore and J.S. Clegg, In: The Cell-Cycle: Gene-Enzyme Interactions, p. 249. Ed. G.M. Padilla, G.L. Whitson and I.L. Cameron Academic Press, New York 1969.
J.S. Clegg, Trans Am. microsc. Soc.93, 481 (1974).
T. Iwasaki, in: Inst. Internat. Froid. Commission C-1, 79–88. Sapporo, Japan 1973–1975.
J.S. Clegg, J. exp. Biol.61, 291 (1974).
S. Brunauer, Physical Adsorption of Gases & Vapors. Princeton University Press, New Jersey 1943.
A.C. Zettlemoyer, E.D. Schweitzer and W.C. Walker, J. Am. Leath. Chem. Ass.41, 253 (1946).
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Supported in part by grant PCM-24037 from the US National Science Foundation.
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Clegg, J.S., Zettlemoyer, A.C. & Hsing, H.H. On the residual water content of dried but viable cells. Experientia 34, 734–735 (1978). https://doi.org/10.1007/BF01947290
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DOI: https://doi.org/10.1007/BF01947290