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On the solvent-extraction of acetylcholine receptor

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Summary

Chloroform/methanol rechromatography on Sephadex LH-20 of fractions isolated from organic solvent extracts ofE. electricus electric organs exhibited alterations in elution point compared to the original chromatography. Additionally,3H decamethonium bromide did not coelute with any of the observed fractions. It is concluded that as presently described the organic solvent isolation procedure for the cholinergic receptor exhibits undesirable characteristics.

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Authors and Affiliations

  1. Environmental Biosciences Department, Naval Medical Research Institute, National Naval Medical Center, 20014, Bethesda, Maryland, USA

    J. P. Christopher, D. B. Millar & Melba A. Grafius

Authors
  1. J. P. Christopher
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  2. D. B. Millar
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  3. Melba A. Grafius
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Additional information

Acknowledgments. The authors thank Dan Burrough and Rick O'Brien for their competent technical assistance. Naval Medical Research Development and Command, Research Work Unit No. MRO41.06.01.0021. The opinions and assertions contained herein are the private ones of the writers and are not to be construed as official or reflecting the views of the Navy Department or the naval service at large.

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Christopher, J.P., Millar, D.B. & Grafius, M.A. On the solvent-extraction of acetylcholine receptor. Experientia 34, 689–691 (1978). https://doi.org/10.1007/BF01947259

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  • DOI: https://doi.org/10.1007/BF01947259

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