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A simple diagnostic method for the differentiation of Tamm-Horsfall glycoproteins from healthy probands and those from recurrent calcium oxalate renal stone formers

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Abstract

Tamm-Horsfall glycoproteins (THPs)* from healthy probands, and those from a majority of recurrent calcium oxalate renal stone formers, reveal different properties when analyzed using isoelectric focusing. The pl-values of THPs from healthy probands are approximately 3.5 while THPs from recurrent renal stone formers have pl-values between 4.5 and 6. The two groups of THPs exhibit completely different protein patterns in IEF. This proves the structural difference of these THPs. The differences in IEF analysis allow the differentiation between THPs from healthy probands and those from recurrent calcium oxalate stone formers. These differences could possibly by used as a simple diagnostic method for the recognition of recurrent calcium oxalate renal stone formers.

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Abbreviations

THP:

Tamm-Horsfall glycoprotein

IEF:

Isoelectric focusing

SDS-PAGE:

Sodium dodecyl sulfate-polyacrylamide gel electrophoresis

TCA:

Trichloroacetic acid

kD:

Kilo Dalton

D:

Dalton

CBB G-250:

Coomassie Brilliant Blue G-250

Tris:

Tris(hydroxymethyl)aminomethane

GalNAc:

N-acetylgalactosamine

TEMED:

N,N,N′,N′-tetramethylethylenediamine

M.W.:

Molecular weight

P:

Power

I:

Current

CDG:

Carbohydrate deficient glycoprotein

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  1. Department of Analytical Chemistry, Institute of Inorganic Chemistry, University of Basel, Spitalstrasse 51, CH-4056, Basel, Switzerland

    P. Schnierle

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  1. P. Schnierle
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Schnierle, P. A simple diagnostic method for the differentiation of Tamm-Horsfall glycoproteins from healthy probands and those from recurrent calcium oxalate renal stone formers. Experientia 51, 1068–1072 (1995). https://doi.org/10.1007/BF01946918

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  • DOI: https://doi.org/10.1007/BF01946918

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