Summary
The effects of the inhibition of steroidogenesis by trilostane on oocyte maturation were examined by studying spontaneous maturation and fertilization in vitro. 10−6 M trilostane had no influence on the meiotic process, whether the oocytes were naked or not. At a concentration of 10−6 M and 10−7 M trilostane, low normal pronuclear formation and high polyspermy were found during in vitro fertilization. However, no retarded male pronuclear development could be detected in the trilostane-treated group. Thus, steroid producing activity within ova is apparently necessary to prevent multiple sperm penetration, but it has no effect on meiosis or the action of the so-called male pronucleus growth factor (MPGF).
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Suzuki, S., Endo, Y., Miura, R. et al. Biological effects of trilostane in vitro on oocyte maturation and fertilization in the hamster. Experientia 40, 1214–1217 (1984). https://doi.org/10.1007/BF01946649
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DOI: https://doi.org/10.1007/BF01946649