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Experientia

, Volume 33, Issue 5, pp 589–591 | Cite as

Some characteristics of urokinase released in organ culture of human kidney

  • B. Åstedt
  • B. Bladh
  • L. Holmberg
Specialia

Summary

Plasminogen activator produced in organ culture of human kidney, i.e. in the histotypical arrangement of the tissue, was partially purified by affinity chromatography on para-aminobenzamidine coupled to Sepharose by a 6-carbon spacer, followed by gel chromatography on Sephadex G-100. The molecular weight of 2 active peaks were 27,000 and 52,000 daltons respectively. It was inhibited by DFP and by IgG antiurokinase.

Keywords

Molecular Weight Chromatography Plasminogen Plasminogen Activator Organ Culture 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 2.
    J. Ploug and N. O. Kjeldgaard, Biochim., biophys. Acta24, 278 (1957).Google Scholar
  2. 3.
    A. Lesuk, L. Terminiello and J. H. Traver, Science147, 880 (1965).Google Scholar
  3. 4.
    W. F. White, G. H. Barlow and M. M. Mozen, Biochemistry5, 2160 (1966).Google Scholar
  4. 5.
    N. Ogawa, H. Yamamoto, T. Katamine and H. Tajima, Thromb. Diath. haemorrh.34, 194 (1975).Google Scholar
  5. 6.
    E. V. Barnett and S. Baron, Proc. Soc. exp. Biol. Med.102, 308 (1959).Google Scholar
  6. 7.
    R. H. Painter and A. F. Charles, Am. J. Physiol.202, 1125 (1962).Google Scholar
  7. 8.
    M. B. Bernik and H. C. Kwaan, J. Lab. clin. Med.70, 650 (1967).Google Scholar
  8. 9.
    M. B. Bernik and H. C. Kwaan, J. clin. Invest.48, 1740 (1969).Google Scholar
  9. 10.
    G. H. Barlow and L. Lazer, Thromb. Res.1, 201 (1972).Google Scholar
  10. 11.
    B. Åstedt and M. Pandolfi, Rev. eur. ét. clin. Biol.17, 743 (1972).Google Scholar
  11. 12.
    I. M. Nilsson and B. Olow, Thromb. Diath. haemorrh.8, 297 (1962).Google Scholar
  12. 13.
    M. Steinbuch and R. Audran, Archs Biochem. Biophys.134, 279 (1969).Google Scholar
  13. 14.
    Ö. Ouchterlony, in: Handbook of experimental immunology, p. 655. Ed. D. M. Weir. Blackwell Scientific Publications, Oxford and Edinburgh 1967.Google Scholar
  14. 15.
    L. Holmberg, B. Bladh and B. Åstedt, Biochim. biophys. Acta445, 215 (1976).Google Scholar
  15. 16.
    H. Landmann and F. Markwardt, Experientia26, 145 (1970).Google Scholar
  16. 17.
    P. Kok and T. Astrup, Biochemistry8, 79 (1969).Google Scholar
  17. 18.
    W. Doleschel and W. Auerswald, Med. Pharmac. exp.16, 225 (1967).Google Scholar
  18. 19.
    A. J. Johnson, M. E. Soberano and E. B. Ong. in: Serano Symposia, p. 21. Milan.Google Scholar

Copyright information

© Birkhäuser-Verlag 1977

Authors and Affiliations

  • B. Åstedt
    • 1
  • B. Bladh
    • 1
  • L. Holmberg
    • 1
  1. 1.Research Laboratories of the Department of Gynecology and Obstetrics, and Department of PediatricsUniversity of LundMalmö(Sweden)

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