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Enzyme immunometric assay for the determination of pregnancy associated plasma protein A (PAPP-A) with the antigen as solid phase (conjoint IEMA)

Experientia volume 40, pages 1022–1024 (1984)Cite this article

Summary

Purified pregnancy associated plasma protein A (PAPP-A) can be effectively bound to polystyrene microtitre plates. This immobilized antigen competes with the added serum PAPP-A of unknown concentration for the limited amount of peroxidase-labeled monospecific anti-PAPP-A antibody incubated simultaneously. The sensitivity is 0.2 WHO unit/ml and non-specific binding is 1.0%.

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References

  1. Lin, T. M., Halbert, S. P., Kiefer, D., Spellacy, W. N., and Gall, S., Am. J. Obstet. Gynec.118 (1974) 223.

    Google Scholar 

  2. Bischof, P., Arch. Gynaek.227 (1979) 315.

    Google Scholar 

  3. Folkersen, J., Grudzinskas, J. G., Hindersson, P., Teisner, B., and Westergaard, J. G., Am. J. Obstet. Gynec.139 (1981) 910.

    Google Scholar 

  4. Bischof, P., Bruce, D., Cunningham, P., and Klopper, A., Clinica chim. Acta95 (1979) 243.

    Google Scholar 

  5. Bischof, P., Haenggeli, L., Sizonenko, M. T., Herrmann, W., and Sizonenko, P. C., Biol. Reprod.24 (1981) 1076.

    Google Scholar 

  6. Sinosich, M. T., Teisner, B., Folkersen, J., Saunders, D. M., and Grudzinskas, J. G., Clin. Chem.28 (1982) 50.

    Google Scholar 

  7. Anthony, F., Masson, G. M., and Wood, P. J., Ann. clin. Biochem.20 (1983) 26.

    Google Scholar 

  8. Pledger, D. R., and Belfield, A., Ann. clin. Biochem.20 (1983) 236.

    Google Scholar 

  9. Bersinger, N., Clinica chim. Acta134 (1983) 297.

    Google Scholar 

  10. Davey, M. W., Teisner, B., Sinosich, M., and Grudzinskas, J. G., Analyt. Biochem.131 (1983) 18.

    Google Scholar 

  11. Lowry, O. H., Rosebrough, N. J., Farr, A. L., and Randall, R. J., J. biol. Chem.193 (1951) 265.

    Google Scholar 

Download references

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Affiliations

  1. Department of Obstetrics and Gynaecology, Royal Infirmary, University of Aberdeen, Foresterhill, AB9 2ZB, Aberdeen, (Scotland)

    N. A. Bersinger

Authors
  1. N. A. Bersinger
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Additional information

Acknowledgments. My thanks go to the Janggen-Pöhn-Stiftung, St. Gallen, Switzerland, for the reception of a fellowship grant which enabled this work to be carried out. I would also like to thank Arnold Klopper, Professor of Reproductive Endocrinology, for many stimulating discussions, Garry Luke for excellent technical assistance, and the Department of Medical Illustration of the University of Aberdeen for the preparation of the graphics. The donkey serum was generously supplied by the Scottish Antibody Production Unit, Carluke, Lanarkshire.

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Bersinger, N.A. Enzyme immunometric assay for the determination of pregnancy associated plasma protein A (PAPP-A) with the antigen as solid phase (conjoint IEMA). Experientia 40, 1022–1024 (1984). https://doi.org/10.1007/BF01946490

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  • DOI: https://doi.org/10.1007/BF01946490

Key words

  • Serum
  • pregnancy
  • placental proteins
  • enzyme immunoassay