Summary
Horse serum (HS) supported growth and differentiation of blastocysts as well as or better than fetal bovine serum (FBS) (p<0.01) as measured by a) the onset of trophoblastic outgrowth, b) the size of the resultant outgrowths, c) the size of nuclei in trophoblasts after their outgrowth and d) the size of the inner cell mass in outgrowths. Moreover, polyamines were found to be approximately 10–100 times less toxic to embryos when added to medium containing HS than when the medium contained FBS.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Sherman, M. I., and Wudl, L. R., in: The Cell Surface in Animal Embryogenesis and Development, p. 81 Eds G. Poste and G. L. Nicolson. Elsevier/North Holland, Amsterdam 1976.
Mclaren, A., in: Regulation of Mammalian Reproduction, p. 321. Eds. S. J. Segal, R. Crozier, P. A. Corf and P. G. Cordliffe. Thomas Publishing Co., Springfield, Illinois 1973.
Alexandre, H., J. Embryol. exp. Morph.53 (1979) 153.
Van Winkle, L. J., and Campione, A. L., J. Reprod. Fert.68 (1983). 437.
Biggers, J. D., Leonov, B. V., Baskar, J. F., and Fried, J., Biol. Reprod.19 (1978) 519.
Kaye, P. L., Schultz, G. A., Johnson, M. H., Pratt, H. P. M., and Church, R. B., J. Reprod. Fert.65 (1982) 367.
Allen, J. C., Smith, C. J., Hussain, J., Thomas, J. M., and Gaugas, J. M., Eur. J. Biochem.102 (1979) 153.
Higgens, M. L., Tillman, M. C., Rupp, J. P., and Leach, F. R., J. Cell Physiol.74 (1969) 149.
Tabor, H., and Tabor, C. W., Adv. Enzymol.36 (1972) 203.
Bachrach, V., Menashe, M., Faber, J., Desser, H., and Seiler, N., Adv. Polyamine Res.3 (1981) 259.
Fowler, R. E., and Edwards, R. G., J. Endocr.15 (1957) 374.
Van Winkle, L. J., J. exp. Zool.202 (1977) 275.
Van Winkle, L. J., J. exp. Zool.218 (1981) 239.
Van Winkle, L. J., Biochem. biophys. Res. Commun.98 (1981) 562.
Van Winkle, L. J., and Campione, A. L., Experientia38 (1982) 354.
Woolf, C. M., Principles of Biometry. D. Van Nostrand, London 1968.
Kramer, C. Y., Biometrics12 (1956) 307.
Seidenfeld, J., Doctoral Dissertation. University of California, San Francisco 1979.
Author information
Authors and Affiliations
Additional information
Acknowledgments. Thanks to Dr David Mann and Mrs Annice Richard for their help in preparing this manuscript. Supported by the Chicago College of Osteopathic Medicine.
Rights and permissions
About this article
Cite this article
Van Winkle, L.J., Campione, A.L. Periimplantation-like growth and development of mouse blastocysts in medium containing horse serum. Experientia 40, 988–990 (1984). https://doi.org/10.1007/BF01946473
Issue Date:
DOI: https://doi.org/10.1007/BF01946473