Summary
A new procedure has been worked out to establish restriction maps. The method is fast, does not in general require labeled DNA and has been applied to map the linear palindromic rDNA ofPhysarum with the restriction enzymeBstEII.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Behrens, K., Seebeck, T., and Braun, R., Preparation of ribosomal DNA, in: Cell Biology ofPhysarum andDidymium, vol. II, pp. 301–306. Eds H. C. Aldrich and J. W. Daniel. Academic Press, New York 1982.
Campbell, G. R., Littau, V. C., Melera, P. W., Allfrey, V. G., and Johnson, E. M., Unique sequence arrangement of ribosomal genes in the palindromic rDNA molecule ofPhysarum polycephalum. Nucl. Acids Res.6 (1979) 1433–1447.
Chen, C. W., and Thomas, C. A. Jr, Recovery of DNA segments from agarose gels. Analyt. Biochem.101 (1980) 339–341.
Chen, C. W., and Thomas, C. A., A rapid two-dimensional-fractionation of DNA restriction fragments. Analyt. Biochem.131 (1983) 397–403.
Ferris, P. J., and Vogt, V. M., Structure of the central spacer region of extrachromosomal ribosomal DNA inPhysarum polycephalum. J. molec. Biol.159 (1982) 359–381.
Glover, D. M., and Hogness, D. S., A novel arrangement of the 18S and 28S sequences in a repeating unit ofDrosophila melanogaster rDNA. Cell10 (1977) 167–176.
Hayward, G. S., and Smith, M. G., The chromosome of bacteriophage T5 I. Analysis of the single-stranded DNA fragments by agarose gel electrophoresis. J. molec. Biol.63 (1972) 388–395.
Molgaard, H. V., Matthews, H. R., and Bradbury, E. M., Organization of genes for ribosomal RNA inPhysarum polycephalum. Eur. J. Biochem.68 (1976) 541–549.
Rigby, P. W. J., Dieckmann, M., Rhodes, C. R., and Berg, P., Labeling deoxyribonucleic acid to high specific activity ‘in vitro’ by nick translation with DNA polymerase I. J. molec. Biol.113 (1977) 237–251.
Smith, H. D., and Birnstiel, M. L., A simple method for DNA restriction site mapping. Nucl. Acids Res.3 (1976) 2378–2398.
Southern, E. M., Detection of specific sequences among DNA fragments separated by gel electrophoresis. J. molec. Biol.98 (1975) 503–517.
Vogt, V. M., and Braun, R., Structure of ribosomal DNA inPhysarum polycephalum. J. molec. Biol.106 (1976) 567–587.
Author information
Authors and Affiliations
Additional information
Acknowledgment. This work was supported by grants from the NIH (GM-30338), the NSF (PCM 8116391) and the Swiss National Science Foundation (3.075.81).
Rights and permissions
About this article
Cite this article
Chen, C.W., Braun, R. & Thomas, C.A. Mapping of restriction enzyme cuts by a new two-dimensional procedure. Experientia 40, 921–923 (1984). https://doi.org/10.1007/BF01946440
Issue Date:
DOI: https://doi.org/10.1007/BF01946440