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Experientia

, Volume 29, Issue 7, pp 820–821 | Cite as

Phenobarbital specific antisera and radioimmunoassay

  • A. Chung
  • S. Y. Kim
  • L. T. Cheng
  • A. Castro
Specialia

Keywords

Barbiturate Carbodiimide Specific Antiserum 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Résumé

On a développé un anticorps d'une grande affinité et spécifité envers le phénobarbital en inoculant des lapins avec une solution de barbiturateprotéide, synthétisée d'acide barbiturique de 5-phényl-5-(4-aminobutyl) et d'albumine de sérum bovin par du carbodiimide. Usant de cet anticorps comme radioimmunoeassai, on peut mesurer jusqu'à 1 picomole de phénobarbital par ml de fluides biologique sans que d'autres barbiturates y opposent une réaction significative.

References

  1. 1.
    S. Spector, J. Pharm. exp. Ther.178, 253 (1971).—F. L. Adler andC. T. Liu, J. Immun.106, 1684 (1971).—S. Spector andC. W. Parker, Science168, 1347 (1970).Google Scholar
  2. 2.
    S. Spector andE. J. Flynn, Science 174, 1036 (1971).Google Scholar
  3. 3.
    A. R. Midgley jr. andG. D. Niswender,Steroids Assay by Competitive Protein Binding (Second Karolinsky Symposia on Research Methods in Reproductive Endocrinology, 1970), vol. 1.Google Scholar
  4. 4.
    Cross reactivity=(The amounts of phenobarbital needed to displace 50% of phenobarbital H3 from binding to the antibody)/ (The amounts of barbiturate needed to displace 50% of phenobarbital-H3 from binding to the antibody).Google Scholar
  5. 5.
    B. H. Wainer, F. W. Fitch, R. M. Rothberg andJ. Fried, Science176, 1143 (1972).Google Scholar
  6. 6.
    M. Sela, Ann. N.Y. Acad. Sci.190, 181 (1971).Google Scholar

Copyright information

© Birkhäuser Verlag 1973

Authors and Affiliations

  • A. Chung
    • 1
  • S. Y. Kim
    • 1
  • L. T. Cheng
    • 1
  • A. Castro
    • 1
  1. 1.United Medical LaboratoriesInc., Endocrine Research UnitPortlandUSA

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