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Insolubilized lactoperoxidase for the [125I]-labelling of proteins

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Zusammenfassung

Antikörperfragmente wurden mittels trägergebundener Lactoperoxidase radioiodiert. Dies erleichterte die Abtrennung des Enzyms vom markierten Protein.

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References

  1. J.J. Marchalonis, Biochem. J.113 299 (1969).—H. Rajaniemi, P. Tuohimaa andM. Niemi, Histochemie23, 342 (1970).—J. I. Thorell andB.G. Johansson, Biochim. biophys. Acta251, 363 (1971).—J.J. Marchalonis, R.E. Cone andV. Santer, Biochem. J.124, 921 (1971).

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  2. Enzacryl AA (Koch-Light Laboratories Ltd., Colnbrook, Bucks., England) is a copolyacrylamide containing aromatic amino groups. A booklet on enzyme insolubilization is available from the manufacturer (R. Epton andT. H. Thomas, An introduction to waterinsoluble enzymes, 1971).

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  5. Eidg. Institut für Reaktorforschung, CH-5303 Würenlingen.

  6. Pharmacia, Uppsala, Sweden.

  7. This work was supported by grant No. 3.709.72 (to C.C.K.) from the Schweizerische Nationalfonds zur Förderung der wissenschaftlichen Forschung.

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Kuenzle, C.C., Döbeli, M. Insolubilized lactoperoxidase for the [125I]-labelling of proteins. Experientia 29, 800–801 (1973). https://doi.org/10.1007/BF01946294

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  • DOI: https://doi.org/10.1007/BF01946294

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