Summary
A method is described to get the secrete of the hatching glands together with the perivitelline fluid by stimulating the eggs ofSalmo gairdneri with dissolved gaseous nitrogen in various buffers. Characterizing this fluid by various methods there could be shown some enzymes of those a proteinase alone seems to be the really ‘hatching enzyme’.
Literatur
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Hagenmaier, H.E. Zum Schlüpfprozess bei Fischen: II. Gewinnung und Charakterisierung des Schlüpfsekretes bei der Regenbogenforelle (Salmo gairdneri Rich.). Experientia 28, 1214–1215 (1972). https://doi.org/10.1007/BF01946178
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DOI: https://doi.org/10.1007/BF01946178