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Heterogeneity of the Fourth International Standard for Insulin by gel-chromatography on sephadex

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Resumen

Se sometieron cristales del Cuarto Patrón Internacional de Insulina a gel-cromatografía por Sephadex. Se determinó que previa a la hormona, eluye una zona de proteínas de mayor peso molecular, cuya posición de elución corresponde a proinsulina y compuestos intermedios. Su área representa un 4.5% del área total. Se demuestra así la heterogeneidad de dichos cristales, y se sugiere que el Quinto Patrón sea sometido a gel-cromatografía durante su preparación. Este procedimiento daría a dicho Patrón una mayor homogeneidad y potencia.

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Authors and Affiliations

  1. Instituto Nacional de Farmacología y Bromatología, Caseros 2161, Buenos Aires, (República Argentina)

    R. L. Rolando & D. Torroba

Authors
  1. R. L. Rolando
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  2. D. Torroba
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Rolando, R.L., Torroba, D. Heterogeneity of the Fourth International Standard for Insulin by gel-chromatography on sephadex. Experientia 28, 1169 (1972). https://doi.org/10.1007/BF01946148

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  • DOI: https://doi.org/10.1007/BF01946148

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