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The enhanced induction of metallothionein by zinc, its half-life in the marine fishPleuronectes platessa, and the influence of stress factors on metallothionein levels

Experientia volume 43, pages 178–181 (1987)Cite this article

Summary

Intraperitoneal injection of zinc raised levels of a hepatic metallothionein-like species. Assuming that this species was metallothionein (MT) then levels were raised from approximately 20 μg/g to 300 μg/g in 7 days, and levels thereafter remained high for the next 4 weeks. The half-lives of the protein in liver and kidney from starved fish, measured using in vivo incorporation of35S cysteine at 11°C, were approximately 27 days and 32 days respectively. The following agents failed to stimulate synthesis of MT in plaice: stress (due to catching), endotoxin, dexamethasone, cortisol and turpentine.

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Affiliations

  1. NERC Institute of Marine Biochemistry, St. Fittick's Road, AB13RA, Aberdeen, Scotland

    J. Overnell, R. McIntosh & T. C. Fletcher

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  1. J. Overnell
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  2. R. McIntosh
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  3. T. C. Fletcher
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Overnell, J., McIntosh, R. & Fletcher, T.C. The enhanced induction of metallothionein by zinc, its half-life in the marine fishPleuronectes platessa, and the influence of stress factors on metallothionein levels. Experientia 43, 178–181 (1987). https://doi.org/10.1007/BF01942842

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  • DOI: https://doi.org/10.1007/BF01942842

Key words

  • Metallothionein induction
  • zinc metallothionein half-life
  • plaice
  • stress
  • fish