Summary
Male mice were injected i.p. with 2.5 mg/kg mitomycin C, 100 mg/kg ethyl nitrosourea or saline and mated with untreated virgin females five weeks later. Sperm from 64 of the F1 male progeny were analyzed histochemically for acrosin, succinic dehydrogenase and alpha-glycerophosphate dehydrogenase activity. The frequency of F1 males with sub-normal sperm enzyme activity was significantly higher among progeny from treated males than in controls. These results show that analysis of sperm enzyme activity in F1 males is a practical method for detection of transmitted mutations induced in a treated parent.
This is a preview of subscription content, log in via an institution to check access.
References
Crow, J. F., Identifying and Estimating the Genetic Impact of Chemical Mutagens. National Academy Press, Washington 1983.
Russel, L. B., and Shelby, M. D., Mutation Res.154 (1985) 69.
Ficsor, G., Ginsberg, L. C., Snoke, R. E., and Becker, R. W., Fert. Steril.39 (1983) 297.
Detailed enzyme assay conditions will be sent on request.
Hrudka, F., Andrology11 (1979) 337.
Ficsor, G., Oldford, G. M., Loughlin, K. R., Panda, B. B., Dubien, J. L., and Ginsberg, L. C., Envir. Mutagen.6 (1984) 297.
Burkhart, J. G., Gay, C. P., and Malling, H. V., Mutation Res.92 (1982) 249.
Hugenholtz, A. P., and Bruce, W. R., Mutation Res.107 (1983) 177.
Bhattacharya, G. K., and Johnson, R. A., Statistical Concepts and Methods, p. 639. Wiley, New York 1977.
Ehling, U. H., in: Chemical Mutagens: Principles and Methods for their Detection, p. 233. Eds A. Hollaender and F. J. deSerres. Plenum, New York 1978.
Russel, L. B., Mutation Res.86 (1981) 329.
Ehling, U. H., Charles, D. J., Favor, J., Graw, J., Kratochvilova, J., Neuhauser-Klaus, A., and Pretsch, W., Mutation Res.150 (1985) 393.
Hecht, N. B., Bower, P. A., Waters, S. H., Yelick, P. C., and Distel, R. J., Exp. Cell Res.164 (1986) 183.
Staub, J. E., and Matter, B. E., Archs Genet.49 (1977) 29.
Kapp, R. W., Picciano, D. J., and Jacobson, C. B., Mutation Res.64 (1979) 47.
Wyrobek, A. J., Gordon, L. A., Burkhart, J. G., Francis, M. W., Kapp, R. W., Letz, G., Malling, H. V., Topham, J. C., and Whorton, M. D., Mutation Res.115 (1983) 1.
Author information
Authors and Affiliations
Additional information
We gratefully acknowledge USPHS, NIEHS grant 1 RO1 ES02607-02 and technical assistance by G. M. Oldford.
Rights and permissions
About this article
Cite this article
Panda, B.B., Ficsor, G. & Ginsberg, L.C. Sperm enzyme activity analysis in individual sperm for detection of heritable mutations in mammals. Experientia 44, 253–254 (1988). https://doi.org/10.1007/BF01941727
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/BF01941727