, Volume 44, Issue 3, pp 251–252 | Cite as

Kinetic arguments for the existence of a single form of intestinal ornithine decarboxylase during the postnatal development of normal and sparse-fur mutant mice and after EGF treatment

Short Communications


The Km for ornithine is remarkably constant during the course of postnatal development in both normal and spf mutant mice even if a large but transient increase in ornithine decarboxylase (ODC) activity is noted. Four hours after EGF injection (4 μg/g b.wt) to 17-day-old normal and spf mice, a marked stimulation of ODC activity is observed but Km remains unaffected. These data argue against the existence of multiple forms of ODC in the intestinal mucosa of mice.

Key words

Ornithine decarboxylase small intestine postnatal development kinetic properties EGF treatment sparse-fur mutant mice 


  1. 2.
    Pegg, A. E., Biochem. J.234 (1986) 249.PubMedGoogle Scholar
  2. 3.
    Tabor, C. W., and Tabor H., A. Rev. Biochem.53 (1984) 749.Google Scholar
  3. 4.
    Kuehn, G. D., and Atmar, V. J., Fedn Proc.41 (1982) 3078.Google Scholar
  4. 5.
    Malo, C., Qureshi, I. A., and Letarte, J., Am. J. Physiol.250 (1986) G177.PubMedGoogle Scholar
  5. 6.
    Luk, G. D., Bayless, T. M., and Baylin, S. B. J. clin. Invest.66 (1980) 66.PubMedGoogle Scholar
  6. 7.
    Luk, G. D., and Baylin, S. B., in: Mechanisms of Intestinal Adaptation, p. 65. Eds J. W. L. Robinson, R. H. Dowling and E. O. Rucken. MTP Press, New York 1981.Google Scholar
  7. 8.
    Lau, C., and Slotkin, T. A., Molec. Pharmac.16 (1979) 504.Google Scholar
  8. 9.
    Obenrader, M. K., and Prouty, W. K., J. biol. Chem.252 (1977) 2860.PubMedGoogle Scholar
  9. 10.
    Seely, J. E., Persson, L., Sertich, G. J., and Pegg, A. E., Biochem. J.226 (1985) 577.PubMedGoogle Scholar
  10. 11.
    Loeb, D., Houben, P. W., and Bullock L. P., Molec. cell. Endocr.38 (1984) 67.PubMedGoogle Scholar
  11. 12.
    Seidel, E. R., Am. J. Physiol.251 (1986) G460.PubMedGoogle Scholar
  12. 13.
    Feldman, E. J., Aures, D., and Grossman, M. I., Proc. Soc. exp. Biol. Med.159 (1978) 400.PubMedGoogle Scholar
  13. 14.
    Malo, C., and Ménard, D., Gastroenterology83 (1982) 28.PubMedGoogle Scholar
  14. 15.
    Eadie, G. S., Science116 (1952) 688.PubMedGoogle Scholar
  15. 16.
    Hofstee, B. H. J., Science116 (1952) 329.PubMedGoogle Scholar
  16. 17.
    Lowry, O. H., Rosebrough, N. F., Farr, A. L., and Randall, R. J., J. biol. Chem.193 (1951) 265.PubMedGoogle Scholar
  17. 18.
    Bachrach, U., Cell Biochem. Function2 (1984) 6.Google Scholar
  18. 19.
    Mitchell, J. L. A., Mitchell, G. K., and Carter, D. D., Biochem. J.205 (1982) 551.PubMedGoogle Scholar
  19. 20.
    Pereira, M. A., Savage, R. E., and Guion, C., Biochem. Pharmac.32 (1983) 2511.Google Scholar

Copyright information

© Birkhäuser Verlag 1988

Authors and Affiliations

  • C. Malo
    • 1
  1. 1.Department of Physiology, Faculty of MedicineUniversity of MontrealMontréalCanada

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