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Sclerotial and low aflatoxigenic morphological variants from haploid and diploidAspergillus parasiticus

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Summary

Serial transfer of mycelial macerates of a wild type, haploid, aflatoxigenic strain ofAspergillus parasiticus in a defined liquid medium resulted in the production of three new morphological classes: a sclerotial form with high aflatoxin production, and two variant forms (fan andfluff) with lowered sporulation, no sclerotia, and attenuated levels of aflatoxin. A genetically marked diploid containing mutant markers for aflatoxin pathway intermediates yielded the same three morphological classes upon serial transfer of macerated mycelia. When these diploid variants were treated with a haploidization agent, and the phenotypes of the resultant segregants scored, a low frequency of colonies producing aflatoxin pathway intermediates was recovered. These genetic data indicate that the structural genes for the aflatoxin pathway are present but somehow attenuated in thefan andfluff strains.

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References

  1. Diener, U. L., and Davis, N. D., Phytopathology56 (1966) 1390.

    CAS  PubMed  Google Scholar 

  2. Mayne, R. Y., Bennett, J. W., and Tallant, J., Mycologia63 (1971) 644.

    Article  CAS  PubMed  Google Scholar 

  3. Lee, E. G.-H., and Townsley, P. M., J. Food Sci.33 (1968) 420.

    Article  CAS  Google Scholar 

  4. Bennett, J. W., J. gen. Microbiol.124 (1981) 429.

    CAS  Google Scholar 

  5. Bennett, J. W., Silverstein, R. B., and Kruger, S. J., J. Am. Oil Chem. Soc.58 (1981) 952A.

    Article  CAS  Google Scholar 

  6. Torres, J., Guarro, J. Suarez, G., Sune, N., Calvo, M. A., and Ramirez, C., Mycopathologia72 (1980) 171.

    Article  CAS  PubMed  Google Scholar 

  7. Papa, K. E., Mycologia65 (1973) 1201.

    Article  CAS  PubMed  Google Scholar 

  8. Papa, K. E., Mycologia70 (1978) 766.

    Article  CAS  PubMed  Google Scholar 

  9. Bennett, J. W., and Christensen, S. B., Adv. appl. Microbiol.29 (1983) 53.

    Article  CAS  PubMed  Google Scholar 

  10. Bennett, J. W., J. gen. Microbiol.113 (1979) 127.

    Article  CAS  PubMed  Google Scholar 

  11. Adye, J., and Mateles, R. I., Biochem. biophys. Acta86 (1964) 135.

    Article  Google Scholar 

  12. Hara, S., Fennell, D. I., and Hesseltine, C. W., Appl. Microbiol.27 (1974) 1118.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  13. Pons, W. A., Cucullu, A. F., Franz, A. O., and Goldblatt, L. A., J. Ass. off. analyt. Chem.45 (1968) 694.

    CAS  Google Scholar 

  14. We are indebted to Dr M. Christensen, Department of Botany, Univ. of Wyoming, Laramie, Wyoming, USA, for taxonomic identification of the variants.

  15. Raper, K. B., and Fennell, D. I., The GenusAspergillus, William & Wilkins. Baltimore 1965.

    Google Scholar 

  16. Christensen, M., Mycologia73 (1981) 1056.

    Article  Google Scholar 

  17. Murakami, H., Hayashi, K., and Ushijima, S. J., Gen. appl. Microbiol.28 (1982) 55.

    Article  Google Scholar 

  18. Bennett, J. W., Wheeler, D. G., and Dunn, J. J., in: Advances in Biotechnology, Vol. 3, Fermentation products, p. 417. Eds M. Moo-Young, C. Vezina and K. Singh. Pergamon, Toronto 1981.

    Chapter  Google Scholar 

  19. Bennett, J. W., in: Overproduction of Microbial Products, p. 549. Eds V. Krumphanzl, B. Sikyta and Z. Vanek, Academic Press, London 1982.

    Google Scholar 

  20. Bennett, J. W., Vinnett, C. H., and Goynes, W. R., Can. J. Microbiol.26 (1980) 706.

    Article  CAS  PubMed  Google Scholar 

  21. Jinks, J. L., Nature174 (1954) 409.

    Article  CAS  PubMed  Google Scholar 

  22. Jinks, J. L., in: The Fungi, Vol. 2. The fungal organism, p. 619. Eds G. C. Ainsworth and A. S. Sussman, Academic Press, New York 1966.

    Google Scholar 

  23. Foster, J. W., Woodruff, H. B., and McDaniel, L. E., J. Bacteriol.46 (1943) 421.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  24. Hansen, H. N., and Snyder, W. C., Science49 (1944) 264.

    Article  Google Scholar 

  25. Whiffen, A. J., and Savage, G. M., J. Bact.53 (1947) 231.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  26. MacDonald, K. D., Nature218 (1968) 371.

    Article  CAS  PubMed  Google Scholar 

  27. Hansen, H. N., Mycologia30 (1938) 442.

    Article  Google Scholar 

  28. Kafer, E., Genetics46 (1961) 1581.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  29. Ball, J., J. gen. Microbiol.66 (1971) 63.

    Article  CAS  PubMed  Google Scholar 

  30. Chang, L. T., and Tuveson, R. W., Appl. Microbiol.30 (1975) 141.

    Article  Google Scholar 

  31. Chater, K. F., in: The Stability of Industrial Organisms, p. 7. Ed. B. E. Kirsop, Commonwealth Mycological Institute Kew 1980.

    Google Scholar 

  32. Turner, G., and Ballance, J., in: Gene Manipulation in Fungi, p. 259. Eds J. W. Bennett and L. S. Lasure. Academic Press, New York 1985.

    Chapter  Google Scholar 

  33. Niehaus, W. G. Jr, and Failla, L., J. expl. Mycol.8 (1984) 80.

    Article  CAS  Google Scholar 

  34. Buchanan, R. L., and Lewis, D. F., Appl. envir. Microbiol.48 (1984) 306.

    Article  CAS  Google Scholar 

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Author notes

  1. Phaik-Mooi Leong

    Present address: Molecular Biology Institute, University of California, 90024, Los Angeles, CA, USA

Authors and Affiliations

  1. Department of Biology, Tulane University, 70118, New Orleans, Louisiana, USA

    J. W. Bennett, Phaik-Mooi Leong, S. Kruger & D. Keyes

Authors
  1. J. W. Bennett
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  2. Phaik-Mooi Leong
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  3. S. Kruger
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  4. D. Keyes
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Additional information

This work was supported by a Cooperative Agreement from the U.S. Department of Agriculture, (58-7B30-3-556).

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Bennett, J.W., Leong, PM., Kruger, S. et al. Sclerotial and low aflatoxigenic morphological variants from haploid and diploidAspergillus parasiticus. Experientia 42, 848–851 (1986). https://doi.org/10.1007/BF01941550

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