, Volume 42, Issue 7, pp 826–828 | Cite as

Increased activity of dipeptidyl peptidase IV in serum of hepatoma-bearing rats coincides with the loss of the enzyme from the hepatoma plasma membrane

  • C. Hanski
  • T. Zimmer
  • R. Gossrau
  • W. Reutter
Short Communications


The specific activity of dipeptidyl peptidase IV (DPPIV E.C. 3.4.14.-) in the plasma membrane of Morris hepatoma 9121 or hepatoma 7777 was 3.5% and 2.9%, respectively, of that in the plasma membrane of rat liver. The enzyme activity in the serum of hepatoma-bearing rats was 141% (hepatoma 91219) and 162% (hepatoma 7777) of the normal value. cytochemical investigation showed that the DPP IV activity was almost completely absent from the hepatoma cell plasma membrane and was not sequestered within these cells. Indirect immunofluorescence staining with a polyclonal antibody directed against DPP IV indicated that the loss of activity was due to the absence of DPP IV molecules in the plasma membrane. The possibility that the enzyme is transferred from the membrane into the serum as a result of structural alterations is discussed.

Key words

Dipeptidyl peptidase IV hepatoma rats cancer markers immunofluorescence cytochemistry 


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Copyright information

© Birkhäuser Verlag Basel 1986

Authors and Affiliations

  • C. Hanski
    • 1
  • T. Zimmer
    • 1
  • R. Gossrau
    • 2
  • W. Reutter
    • 1
  1. 1.Institut für Molekularbiologie und Biochemie der Freien Universität BerlinBerlin 33 (Dahlem)
  2. 2.Institut für Anatomie der Freien Universität BerlinBerlin 33 (Dahlem)

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