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Immunocytochemical and enzymatic detection of lysozyme in human colon carcinoma cell lines

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Summary

Six of a total of 14 human colon carcinoma cell lines produce and secrete lysozyme in vitro. Three also produce the enzyme when propagated in vivo in athymic mice. None of the lysozyme positive cells stained in a manner typical of Paneth cells. Additionally, lysozymes from all six colon lines possess identical molecular weights (∼14,000 daltons).

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Authors and Affiliations

  1. Center for Biochemical and Biophysical Sciences and Medicine, Harvard Medical School, and Brigham and Women's Hospital, 250 Longwood Avenue, 02115, Boston, Massachusetts, USA

    J. W. Fett, R. R. Lobb, E. M. Alderman & M. E. Key

  2. Department of Surgery, School of Medicine, State University of New York at Stony Brook, 11794, Long Island, New York, USA

    M. E. Key

Authors
  1. J. W. Fett
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  2. R. R. Lobb
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  3. E. M. Alderman
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  4. M. E. Key
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Additional information

This work was supported by funds from the Monsanto Company under agreements with Harvard University. We are indebted to A. Ehrlich, S. Kane, and D. Sleeman for excellent technical assistance and to Dr B. Vallee for continued advice and support.

J.W.F. and E.M.A. are also members of the Department of Pathology, Harvard Medical School.

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Fett, J.W., Lobb, R.R., Alderman, E.M. et al. Immunocytochemical and enzymatic detection of lysozyme in human colon carcinoma cell lines. Experientia 42, 1159–1162 (1986). https://doi.org/10.1007/BF01941292

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  • DOI: https://doi.org/10.1007/BF01941292

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