Experientia

, Volume 51, Issue 7, pp 725–730 | Cite as

Leishmania donovani in hamsters: Stimulation of non-specific resistance by novel lipopeptides and their effect in antileishmanial therapy

  • K. Zehra
  • R. Pal
  • Anuradha
  • S. Y. Rizvi
  • W. Haq
  • B. Kundu
  • J. C. Katiyar
  • K. B. Mathur
Article

Abstract

Several novel type of lipopeptides were synthesized and evaluated for their ability to stimulate non-specific resistance againstLeishmania donovani infection. Peritoneal macrophages isolated from young male hamsters treated with muramyl dipeptide (MDP) and various synthetic lipopeptides (6 mg/kg i.p.) 7 days earlier, were cultured in vitro and challenged 24 h later withL. donovani promastigotes. One lipopeptide, Central Drug Research Institute (CDRI) compound 86/450, exhibited significantly higher immunostimulatory activity than MDP. Its prophylactic activity was further confirmed in hamsters by giving 2 split doses of 3 mg/kg of the compound spaced at 2 weeks, i.e. on day −7 and +7 of challenge withL. donovani amastigotes. The prophylactic effect lasted for 7 days following the last treatment with compound 86/450. The antileishmanial action of sodium stibogluconate (SAG) was also found to be enhanced by 16% in hamsters primed with compound 86/450.

Key words

Hamsters L. donovani CDRI compound 86/450 muramyl dipeptide (MDP) sodium stibogluconate (SAG) 

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Copyright information

© Birkhäuser Verlag 1995

Authors and Affiliations

  • K. Zehra
    • 1
  • R. Pal
    • 1
  • Anuradha
    • 1
  • S. Y. Rizvi
    • 2
  • W. Haq
    • 2
  • B. Kundu
    • 2
  • J. C. Katiyar
    • 1
  • K. B. Mathur
    • 1
  1. 1.Division of ParasitologyCentral Drug Research InstituteLucknowIndia
  2. 2.Division of BiopolymersCentral Drug Research InstituteLucknowIndia

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