The Journal of Membrane Biology

, Volume 21, Issue 1, pp 125–146 | Cite as

Regulation of cellular calcium metabolism and calcium transport by calcitonin

  • André B. Borle


Calcitonin was studied in isolated kidney cells and in isolated mitochondria. A concentration of 10 ng/ml of synthetic calcitonin increases the cellular accumulation of45Ca and the total cell calcium. The mitochondrial pool is increased several-fold. Kinetic analysis of the data shows that although the total cellular exchangeable calcium pool is enlarged, calcium influx and efflux are significantly depressed by calcitonin. The absence of phosphate or the presence of inhibitors of mitochondrial calcium transport completely abolish the effects of the hormone. In isolated mitochondria, the hormone stimulates the active calcium uptake and depresses the extramitochondrial calcium activity. Calcitonin counteracts the effects of cyclic AMP which stimulates the release of calcium from mitochondria and increases the extramitochondrial calcium activity. These data indicate that cellular calcium homeostasis is controlled by the mitochondrial calcium turnover. They suggest that calcitonin regulates the cell calcium metabolism and inhibits the transcellular calcium transport by stimulating the rate of calcium uptake by mitochondria which depresses cytoplasmic calcium activity.


Calcitonin Calcium Uptake Calcium Metabolism Calcium Transport Cell Calcium 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Aliapoulios, M. A., Goldhaber, P., Munson, P. L. 1966. Thyrocalcitonin inhibition of bone resorption induced by parathyroid hormone in tissue culture.Science 151:330PubMedGoogle Scholar
  2. Barlet, J. P. 1972. Effect of porcine, salmon and human calcitonin on urinary excretion of some electrolytes in sheep.J. Endocrinol. 55:153PubMedGoogle Scholar
  3. Barlet, J. P., Care, A. D. 1972. The influence of parathyroid hormone on urinary excretion of calcium, magnesium, inorganic phosphorus in sheep.Horm. Metab. Res. 4:315PubMedGoogle Scholar
  4. Birnbaumer, L., Pohl, S. L., Rodbell, M. 1969. Adenyl cyclase in fat cells. I. Properties and the effect of adrenocorticotropin and fluoride.J. Biol. Chem. 244:3468PubMedGoogle Scholar
  5. Borle, A. B. 1969. Effects of thyrocalcitonin on calcium transport in kidney cells.Endocrinology 85:194PubMedGoogle Scholar
  6. Borle, A. B. 1970. Kinetic analyses of calcium movements in cells cultures. III. Effects of calcium and parathyroid hormone in kidney cells.J. Gen. Physiol. 55:163PubMedGoogle Scholar
  7. Borle, A. B. 1972. Kinetic analysis of calcium movements in cell culture. V. Intracellular calcium distribution in kidney cells.J. Membrane Biol. 10:45Google Scholar
  8. Borle, A. B. 1973. Calcium metabolism at the cellular level.Fed. Proc. 32:1944Google Scholar
  9. Borle, A. B. 1974a. Cyclic AMP stimulation of calcium efflux from kidney, liver, and heart mitochondria.J. Membrane Biol. 16:221Google Scholar
  10. Borle, A. B. 1974b. Methods for assessing hormone effects of calcium fluxes in vitro.In: Methods in Enzymology. B. O'Malley and J. Hardman, editors. Vol. 39. Academic Press Inc., New York (in press)Google Scholar
  11. Carafoli, E., Lehninger, A. L. 1971. A survey of the interaction of calcium ions with mitochondria from different tissues and species.Biochem. J. 122:681PubMedGoogle Scholar
  12. Chase, L. R., Fedak, S. A., Aurbach, G. D. 1969. Activation of skeletal adenyl cyclase by parathyroid hormone in vitro.Endocrinology 84:761PubMedGoogle Scholar
  13. Copp, D. H. 1973. Calcitonin physiology: Hormonal effects.In: Peptide Hormones. S. A. Berson and R. S. Yalow, editors. p. 999. American Elsevier Publishing Company, Inc., New YorkGoogle Scholar
  14. Drummond, G. I., Duncan, L. 1970. Adenyl cyclase in cardiac tissue.J. Biol. Chem. 245:976PubMedGoogle Scholar
  15. Dufresne, L. R., Gitelman, H. 1972. A possible role of adenyl cyclase in the regulation of parathyroid activity by calcium.In: Calcium, Parathyroid Hormone and the Calcitonins. R. V. Talmage and P. L. Munson, editors. p. 202. Excerpta Medica, AmsterdamGoogle Scholar
  16. Friedman, J., Au, W. Y. W., Raisz, L. G. 1968. Response of fetal rat bone to thyrocalcitonin in tissue culture.Endocrinology 82:149Google Scholar
  17. Friedman, J., Raisz, L. G. 1965. Thyrocalcitonin: Inhibitor of bone resorption in tissue culture.Science 150:1465PubMedGoogle Scholar
  18. Harrell, A., Binderman, I., Rodan, G. A. 1973. The effect of calcium concentration on calcium uptake by bone cells treated with thyrocalcitonin (TCT) hormone.Endocrinology 92:550PubMedGoogle Scholar
  19. Heersche, J. N. M., Marcus, R., Aurbach, G. D. 1974. Calcitonin and the formation of 3′, 5′-AMP in bone and kidney.Endocrinology 94:241PubMedGoogle Scholar
  20. Hirsch, P. F. 1968. Enhancement of hypocalcemic activity of thyrocalcitonin by inorganic phosphate.In: Calcitonin: Proc. Symp. Thyrocalcitonin C Cells. S. Taylor, editor. p. 11. Springer-Verlag, New YorkGoogle Scholar
  21. Hirsch, P. F., Sliwowski, A., Orimo, H., Darago, L. S., Mewborn, Q. A., Jr. (1973). On the mode of the hypocalcemic action of thyrocalcitonin and its enhancement by phosphate in rats.Endocrinology 93: 12PubMedGoogle Scholar
  22. Huxley, A. F. 1960. A. K. Solomon: Compartmental Methods of Kinetic Analyses (Appendix).In: Mineral Metabolism. C. L. Comar and F. Bronner, editors. Vol. 1, Pt. A., p. 119. Academic Press, New YorkGoogle Scholar
  23. Kennedy, J. W., III, Tanzer, F. S., Talmage, R. V. 1969. Plasma phosphate and the hypocalcemic response of intact, parathyroidectomized and nephrectomized rats to thyrocalcitonin.Endocrinology 85: 657PubMedGoogle Scholar
  24. Lehninger, A. L. 1970. Mitochondria and calcium transport.Biochem. J. 119: 129PubMedGoogle Scholar
  25. Lehninger, A. L., Carafoli, E., Rossi, C. S. 1967. Energy-linked ion movements in mitochondrial systems.Advanc. Enzymol. 29: 259Google Scholar
  26. MacManus, J. P., Whitfield, J. F. 1970. Inhibition by thyrocalcitonin of the mitogenic action of parathyroid hormone and cyclic 2′, 5′ monophosphate on rat thymocytes.Endocrinology 86: 934PubMedGoogle Scholar
  27. Marcus, R., Aurbach, G. D. 1971. Adenyl cyclase from renal cortex.Biochim. Biophys. Acta 242: 410PubMedGoogle Scholar
  28. Matthews, J. L., Martin, J. H., Collins, E. J., Kennedy, J. W., III, Powell, E. L., Jr. 1972. Immediate changes in the ultrastructure of bone cells following thyrocalcitonin administration.In: Calcium Parathyroid Hormone and the Calcitonins. R. V. Talmage and P. L. Munson, editors. p. 375. Excerpta Medica, AmsterdamGoogle Scholar
  29. Melson, G. L., Chase, L. R., Aurbach, G. D. 1970. Parathyroid hormone-sensitive adenyl-cyclase in isolated renal tubules.Endocrinology 86: 511PubMedGoogle Scholar
  30. Murad, F., Brewer, H. B., Vaughan, M. 1970. Effect of thyrocalcitonin on adenosine 3′,5′-cyclic phosphate formation by rat kidney and bone.Proc. Nat. Acad. Sci. 65: 466Google Scholar
  31. Nisket, J., Nordin, B. E. C. 1968. Thyrocalcitonin inhibition of bone resorption in tissue culture.In: Calcitonin. Proc. Symp. Thyrocalcitonin C Cells. S. Taylor, editor. p. 230. Springer-Verlag, New YorkGoogle Scholar
  32. Nordquist, R. E., Palmieri, G. M. A. 1974. Intracellular localization of parathyroid hormone in the kidney.Endocrinology 95: 229PubMedGoogle Scholar
  33. Olson, E. B., Jr., DeLuca, H. F., Potts, J. T. 1972. Calcitonin inhibition of vitamin D induced intestinal calcium absorption.Endocrinology 90: 151PubMedGoogle Scholar
  34. Orimo, H., Fujita, T., Yoshikawa, M. 1969. Pyrophosphate enhancement of hypocalcemic effect of thyrocalcitonin in rat.Endocrin. Japon 16: 303Google Scholar
  35. Pak, C. Y. C., Ruskin, B., Casper, A. 1970. Renal effects of porcine thyrocalcitonin in the dog.Endocrinology 87: 262PubMedGoogle Scholar
  36. Parkinson, D. L., Radde, I. C. 1970. Calcitonin action on membrane ATPase, a hypothesis.In: Calcitonin. 1969 Proceedings of the Second International Symposium. S. Taylor and G. Foster, editors. p. 466. Springer-Verlag, New YorkGoogle Scholar
  37. Pechet, M. M., Bobadilla, E., Carroll, E. L., Hesse, R. H. 1967. Regulation of bone resorption and formation. Influences of thyrocalcitonin, parathyroid hormone neutral phosphate and vitamina D3.Amer. J. Med. 43: 696PubMedGoogle Scholar
  38. Raisz, L. G., Niemann, I. 1969. Effect of phosphate, calcium and magnesium on bone resorption and hormonal respones in tissue culture.Endocrinology 85: 446PubMedGoogle Scholar
  39. Rasmussen, H. 1971. Ionic and hormonal control of calcium homeostasis.Amer. J. Med. 50: 567PubMedGoogle Scholar
  40. Rasmussen, H., Bordier, P. 1974. The Physiological and Cellular Basis of Metabolic Bone Disease. The Williams and Wilkins Company, Baltimore, Md.Google Scholar
  41. Rasmussen, H., Kurokawa, K., DeLong, A. 1971. Phosphate and bone cell function.In: Phosphate et Metabolisme Phosphocalcique. D. J. Hioco, editor. p. 7. Expansion Scientifique Francaise, ParisGoogle Scholar
  42. Rodan, S. B., Rodan, G. A. 1974. The effect of parathyroid hormone and thyrocalcitonin on the accumulation of cyclic adenosine 3′, 5′-monophosphate in freshly isolated bone cells.J. Biol. Chem. 249: 3068PubMedGoogle Scholar
  43. Streeto, J. M. 1969. Renal cortical adenyl cyclase: Effect of parathyroid hormone and calcium.Metabolism 18: 968PubMedGoogle Scholar
  44. Swaminathan, R., Ker, J., Care, A. D. 1974. Calcitonin and intestinal calcium absorption.J. Endocrinol. 61: 83PubMedGoogle Scholar
  45. Wener, J. A., Gorton, S. J., Raisz, L. G. 1972. Escape from inhibition of resorption in cultures of fetal bone treated with calcitonin and parathyroid hormone.Endocrinology 90: 752PubMedGoogle Scholar
  46. Whitfield, J. F., MacManus, J. P., Gillan, D. J. 1971. Inhibition by thyrocalcitonin (calcitonin) of the cyclic AMP-mediated stimulation of thymocytes proliferation by epinephrine.Horm. Metab. Res. 3: 348PubMedGoogle Scholar

Copyright information

© Springer-Verlag New York Inc. 1975

Authors and Affiliations

  • André B. Borle
    • 1
  1. 1.Department of PhysiologyUniversity of Pittsburgh, School of MedicinePittsburgh

Personalised recommendations