The Journal of Membrane Biology

, Volume 37, Issue 1, pp 1–12 | Cite as

Variation in selectivity of univalent cations in slime moldPhysarum polycephalum caused by reception of polyvalent cations

  • Kazuyuki Terayama
  • Kenzo Kurihara
  • Yonosuke Kobatake
Article

Summary

Specificity of reception on 1∶1 electrolytes in the slime moldPhysarum polycephalum was investigated in the presence of polyvalent cations in media. Membrane potential and motive force of tactic movement were examined with the aid of the double chamber method, and the zeta potential at the membrane surface of the slime mold was measured by electrophoretic mobility. The results obtained are summarized as follows: (1) The presence of polyvalent cations (e.g., Ca2+, Mg2+, Sr2+, Ba2+, La3+, Th4+) in medium led to an increase in threshold concentration,C th , determined from the potential measurements for Na- or Li-salts, and to a decrease inC th for K-, Rb-, or NH4-salts,C th for 1∶1 electrolytes changed discontinuously when the concentration of polyvalent cations in medium exceeded their respective thresholds. (2) TheC th determined from chemotaxis agreed with that from the potential response both in the presence and absence of polyvalent cations. (3) Sequence of selectivity of univalent cations varied extensively in the presence of polyvalent cations. (4) Changes in the zeta potential induced by NaCl reception agreed with those in the membrane potential even in the presence of Ca2+ in medium. (5) TheC th for reception of NaCl changed sharply at about 12 °C in the presence of polyvalent cations, while that for KCl was independent of the temperature.

Conformational changes in surface membrane of the slime mold in response to reception of polyvalent cations were then discussed in relation to the discrimination of univalent cations.

Keywords

Membrane Potential Human Physiology Conformational Change Zeta Potential Membrane Surface 

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References

  1. 1.
    Anderson, J.D. 1964. Regional differences in ion concentration in migrating plasmodia.In: Primitive Motile Systems in Cell Biology. D. Allen and N. Kamiya, editors. pp 125–134. Academic Press, New York and LondonGoogle Scholar
  2. 2.
    Camp, W.G. 1936. A method of cultivating myxomycete plasmodia.Bull. Torrey Bot. Club. 63:205Google Scholar
  3. 3.
    Carlilie, M.J. 1970. Nutrition and chemotaxis in the myxomycetePhysarum polycephalum: The effect of carbohydrate on the plasmodium.J. Gen. Physiol. 63:221Google Scholar
  4. 4.
    Coman, D.R. 1940. Additional observation on positive and negative chemotaxis: Experiments with myxomycete.Arch. Pathol. Lab. Med. 29:220Google Scholar
  5. 5.
    Daniel, J.W., Rusch, H.P. 1961. The pure culture ofPhysarum polycephalum on a partial defined soluble medium.J. Gen. Microbiol. 25:47PubMedGoogle Scholar
  6. 6.
    Diamond, J.M., Wright, E.M. 1969. Biomembranes; the physical basis of ion and nonelectrolyte selectivity.Annu. Rev. Physiol. 31:581PubMedGoogle Scholar
  7. 7.
    Eisenman, G. 1962. Cation selective glass electrodes and their mode of operation.Biophys. J. 2:(2):259PubMedGoogle Scholar
  8. 8.
    Eisenman, G. 1969. Theory of membrane electrode potentials: An examination of the parameters determining the selectivity of solid and liquid ion exchangers and of neural ion-sequestering molecules.In: Ion Selective Electrodes. R.A. Durst, editor. pp. 1–56. Nat. Bur. Standards, WashingtonGoogle Scholar
  9. 9.
    Harris, H. 1961. Chemotaxis.Exp. Cell Res. 8(Suppl.):199CrossRefPubMedGoogle Scholar
  10. 10.
    Hato, M., Ueda, T., Kurihara, K., Kobatake, Y. 1976. Change in zeta potential and membrane potential of slime moldPhysarum polycephalum in response to chemical stimuli.Biochim. Biophys. Acta 426:73PubMedGoogle Scholar
  11. 11.
    Inoue, I., Ishida, N., Kobatake, Y. 1973. Studies of excitable membrane formed on the surface of protoplasmic droplets isolated fromNitella: IV. Excitability of the drop membrane in various compositions of the external solutions.Biochim. Biophys. Acta 330:27PubMedGoogle Scholar
  12. 12.
    Kamiya, N. 1942. Physical aspects of protoplasmic streaming.In: The Structure of Protoplasm. W. Seifritz, editor. pp. 199–244. Iowa State College Press, AmesGoogle Scholar
  13. 13.
    Kamiya, N. 1959. Protoplasmic Streaming.Protoplasmatologia 8:1Google Scholar
  14. 14.
    Kashiwagura, T., Kamo, N., Kurihara, K., Kobatake, Y. 1977. Enhancement of salt responses in frog gustatory nerve by removal of Ca2+ from the receptor membrane treated with 1-anilinonaphthalene-8-sulfonate.J. Membrane Biol. 35:205CrossRefGoogle Scholar
  15. 15.
    Katsuki, Y., Hashimoto, T., Yanagisawa, K. 1970. The lateral-line organ of shark as a chemoreceptor.Adv. Biophys. 1:1PubMedGoogle Scholar
  16. 16.
    Kobatake, Y., Inoue, I., Ueda, T. 1975. Physical chemistry of excitable membranes.Adv. Biophys. 7:43PubMedGoogle Scholar
  17. 17.
    Kusano, K. 1958. The influence of cations on the activity of gustatory receptors: II. Effects of NaCl, LiCl, NH4Cl and CsCl.Kumamoto Med. J. 11:240Google Scholar
  18. 18.
    Rosen, W.G. 1962. Cellular chemotropism and chemotaxis.Q. Rev. Biol. 37:242CrossRefPubMedGoogle Scholar
  19. 19.
    Tasaki, I. 1968. Nerve Excitation. C. Thomas, Springfield, Ill.Google Scholar
  20. 20.
    Tasaki, I., Singer, I., Takenaka, T. 1965. Effects of internal and external ionic environment on excitability of squid giant axon.J. Gen. Physiol. 48:1095PubMedGoogle Scholar
  21. 21.
    Terayama, K., Ueda, T., Kurihara, K., Kobatake, Y. 1977. Effect of sugars on salt reception in true slime moldPhysarum polycephalum: Physicochemical interpretation of interaction between salt and sugar receptions.J. Membrane Biol. 34:369CrossRefGoogle Scholar
  22. 22.
    Ueda, T., Muratsugu, M., Kurihara, K., Kobatake, Y. 1976. Chemotaxis inPhysarum polycephalum: Effects of chemicals on isometric tension of the plasmodial strand in relation to chemotactic movement.Exp. Cell Res. 100:337PubMedGoogle Scholar
  23. 23.
    Ueda, T., Terayama, K., Kurihara, K., Kobatake, Y. 1975. Threshold phenomena in chemoreception and taxis by slime moldPhysarum polycephalum.J. Gen. Physiol. 65:223PubMedGoogle Scholar
  24. 24.
    Voet, A. 1939. Quantitative lyotropy.Chem. Rev. 20:169CrossRefGoogle Scholar

Copyright information

© Springer-Verlag New York Inc 1977

Authors and Affiliations

  • Kazuyuki Terayama
    • 1
  • Kenzo Kurihara
    • 1
  • Yonosuke Kobatake
    • 1
  1. 1.Faculty of Pharmaceutical SciencesHokkaido UniversitySapporoJapan

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