, Volume 32, Issue 3, pp 395–396 | Cite as

Studies in relation to endocrine exophthalmos: The biochemical composition of human retrobulbar connective tissue

  • Sant P. Singh
  • Mildred Nikiforuk


As part of studies on the pathogenesis of exophthalmos of Graves' disease, the biochemical composition of human retrobulbar tissue was intestigated. The connective tissue was composed of 72.7% lipid, 23.8% water and 3.5% dried defatted tissue. Total tissue glycosaminoglycan (GAG) amounted to 0.18% of dried defatted tissue. Approximately 27% of the total hexosamine and 19% of the total tissue galactosamine were recovered in the GAG fraction. Cellulose microcolumn fractionation of GAG showed that the hyaluronic acid and dermatan sulfate were the two major GAG species.


Lipid Cellulose Fractionation Connective Tissue Hyaluronic Acid 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


  1. 2.
    D. A. McGill andS. P. Asper, New Engl. J. Med.267, 188 (1962).Google Scholar
  2. 3.
    B. M. Dobyns, inThe Pituitary Gland (Butterworth, London 1966), p. 411.Google Scholar
  3. 4.
    S. P. Singh andJ. M. McKenzie, Metabolism20, 422 (1971).CrossRefPubMedGoogle Scholar
  4. 5.
    C. Cessi andF. Pillego, Biochem. J.77, 508 (1960).PubMedGoogle Scholar
  5. 6.
    C. Cessi andF. Serafini-Cessi, Biochem. J.88, 132 (1963).PubMedGoogle Scholar
  6. 7.
    J. Svejcar andW. B. Robertson, Analyt. Biochem.18, 333 (1967).CrossRefGoogle Scholar
  7. 8.
    S. P. Singh andJ. M. McKenzie, Endocrinology85, 952 (1969).PubMedGoogle Scholar
  8. 9.
    T. Bitter andH. M. Muir, Analyt. Biochem.4, 330 (1962).CrossRefPubMedGoogle Scholar
  9. 10.
    G. Prame, S. Gardell andC. A. Antonopoulos, Expl Eye Res.5, 79 (1966).Google Scholar

Copyright information

© Birkhäuser Verlag 1976

Authors and Affiliations

  • Sant P. Singh
    • 1
  • Mildred Nikiforuk
    • 1
  1. 1.Medical Research ServiceVeterans Administration Hospital and Department of Medicine, Chicago Medical SchoolDowneyUSA

Personalised recommendations