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Experientia

, Volume 42, Issue 9, pp 1016–1018 | Cite as

Determination of erythrocyte pyrimidine 5′-nucleotidase activity by31P nuclear magnetic resonance: Comparison of normal controls and multiple sclerosis patients

  • G. L. Mendz
  • C. R. Middlehurst
  • P. W. Kuchel
  • P. A. Crossie
Short Communications

Summary

A technique to assay erythrocyte pyrimidine 5′-nucleotidase activity in situ using31P nuclear magnetic resonance spectroscopy is presented. The assay is chemically specific, simple and applicable to untreated lysates. A comparison of enzyme levels in normal controls and in multiple sclerosis patients employing the assay yielded no significant differences between both groups. Difficulties encountered in the quantitative analysis of the assay using1H-NMR spectroscopy are briefly discussed.

Key words

Pyrimidine 5′-nucleotidase 31P NMR multiple sclerosis erythrocyte 

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Copyright information

© Birkhäuser Verlag Basel 1986

Authors and Affiliations

  • G. L. Mendz
    • 1
  • C. R. Middlehurst
    • 1
  • P. W. Kuchel
    • 1
  • P. A. Crossie
    • 2
  1. 1.Department of BiochemistryUniversity of SydneySydneyAustralia
  2. 2.Department of MedicineUniversity of SydneySydneyAustralia

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