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ATP-Dependent calcium uptake by cholinergic synaptic vesicles isolated fromtorpedo electric organ

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Summary

Cholinergic synaptic vesicles were purified fromTorpedo electric organ to near morphological homogeneity. They were isolated in a K+ environment. A method is described for the preparation of concentrated synaptic vesicles that allows uptake studies by conventional techniques. An ATP-Mg-dependent calcium uptake associated with synaptic vesicles is characterized. The uptake system transports calcium against a high concentration gradient. The maximum accumulation rate is obtained for the calcium, Mg++ and ATP concentrations likely to be found in the nerve terminal cytoplasm. It is suggested that synaptic vesicles are implicated in the removal of the calcium entering the nerve terminal during synaptic activity.

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Author notes

  1. J. Marsal

    Present address: Facultad de Medicina, Laboratorio de Histología, Departamento de Anatomía Patológica, C.S. “Principes de España” Hospitalet de Llobregat, Universidad de Barcelona, Barcelona, Spain

Authors and Affiliations

  1. Département de Neurochimie, Laboratoire de Neurobiologie Cellulaire, 91190, Gif sur Yvette, France

    M. Israël, R. Manaranche, J. Marsal, F. M. Meunier, N. Morel, P. Frachon & B. Lesbats

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  1. M. Israël
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  2. R. Manaranche
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  6. P. Frachon
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  7. B. Lesbats
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Israël, M., Manaranche, R., Marsal, J. et al. ATP-Dependent calcium uptake by cholinergic synaptic vesicles isolated fromtorpedo electric organ. J. Membrain Biol. 54, 115–126 (1980). https://doi.org/10.1007/BF01940565

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  • DOI: https://doi.org/10.1007/BF01940565

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