, Volume 43, Issue 4, pp 426–428 | Cite as

Cross-reactive monoclonal antibodies to alcohol dehydrogenases

  • B. G. Talbot
  • G. Bilodeau
Short Communications


Three anti-horse liver alcohol dehydrogenase (HLADH) monoclonal antibodies are described. Two are specific for ADH and cross-react with class I and II enzymes from mouse, horse and Chinese hamster. They are specific for the native enzyme but do not inhibit enzyme activity except when combined at high concentration. The third antibody was isolated as a response to rabbit metallothionein. It binds metalloproteins and inhibits ADH activity.

Key words

Monoclonal antibodies alcohol dehydrogenase 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Strydom, D.J., and Vallee, B.J., Analyt. Biochem.123 (1982) 422.CrossRefPubMedGoogle Scholar
  2. 2.
    Vallee, B.J., and Bazzone, T.J., Curr. Top. biol. med. Res.8 (1983) 219.Google Scholar
  3. 3.
    Talbot, B.G., and Thirion, J.P., Genetics88 (1978) 343.PubMedGoogle Scholar
  4. 4.
    Adinolfi, A., Adinolfi, M., and Hupkinson, P.A., Ann. hum. Genet.48 (1984) 1.PubMedGoogle Scholar
  5. 5.
    Beisswenger, T.B., Holmquist, B., and Vallee, B.L., Proc. natn. Acad. Sci.182 (1985) 8369.Google Scholar
  6. 6.
    Talbot, B.G., Qureshi, A.A., Cohen, R., and Thirion J.P., Biochem. Gen.19 (1981) 7.Google Scholar
  7. 7.
    Kennet, R.H., McKeern, T.J., and Bechtol, K.B., Monoclonal Antibodies, Hybridomas. Plenum Press, New York 1980.Google Scholar
  8. 8.
    Talbot, B.G., Maclean, S.J., and Gibson, D.J., Immun. Meth.79, (1985) 283.CrossRefGoogle Scholar
  9. 9.
    Bosron, W.F., Li, T.K., and Vallee, B.L., BBRC91 (1929) 1549.Google Scholar
  10. 10.
    Talbot, B.G., Bilodeau, G., and Thirion, J.P., Molec. Immun.23 (1986) 1133.CrossRefPubMedGoogle Scholar
  11. 11.
    Lad, P.J., Schenk D.B., and Leffert, H.L., Archs Biochem. Biophys.235 (1984) 589.CrossRefGoogle Scholar

Copyright information

© Birkhäuser Verlag 1987

Authors and Affiliations

  • B. G. Talbot
    • 1
  • G. Bilodeau
    • 1
  1. 1.Département de biologie, Faculté des sciencesUniversité de SherbrookeSherbrookeCanada

Personalised recommendations