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In vitro proliferation of human large granular lymphocytes withv-raf/v-myc recombinant retrovirus

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Summary

The effect of infection with a retrovirus carryingv-raf/v-myc oncogenes (J2 virus) on the in vitro proliferation of human large granular lymphocytes (LGL) was investigated. LGL infected with J2 virus (J2LGL), unlike uninfected cells, grew with a proliferation peak eight days after infection. Such cells retained the morphology and functional properties typical of LGL. Furthermore, 5% of J2LGL produced virus the day after infection, whereas non-virus production was detectable five days later. These data indicate that J2 virus provides a transient mitogenic signal for LGL.

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Authors and Affiliations

  1. Laboratory of Experimental Immunology Biological Response Modifiers Program, DCT, NCI-FCRF, Frederick, Maryland, USA

    S. Peppoloni & J. R. Ortaldo

  2. Laboratory of Viral Carcinogenesis Biological Response Modifiers Program, DCT, NCI-FCRF, Frederick, Maryland, USA

    U. R. Rapp

  3. Laboratory of Molecular Immunoregulation, Biological Response Modifiers Program, DCT, NCI-FCRF, Frederick, Maryland, USA

    L. Varesio

  4. Microbiology Section Dept. Experimental Medicine and Biochemical Sciences, University of Perugia, Perugia, Italy

    E. Blasi

  5. Pharmacology Section, Dept. Experimental Medicine and Biochemical Sciences, University of Perugia, Perugia, Italy

    C. Riccardi

  6. Laboratory of Immunopharmacology, SCLAVO Research Center, Via Fiorentina 1, I-53100, Siena, Italy

    S. Peppoloni

Authors
  1. S. Peppoloni
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  2. E. Blasi
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  3. J. R. Ortaldo
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  4. U. R. Rapp
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  5. C. Riccardi
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  6. L. Varesio
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Additional information

Acknowledgments. This work was in part supported by the ‘Associazione Italiana per la Ricerca sul Cancro’ Milan, Italy.

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Peppoloni, S., Blasi, E., Ortaldo, J.R. et al. In vitro proliferation of human large granular lymphocytes withv-raf/v-myc recombinant retrovirus. Experientia 44, 1013–1015 (1988). https://doi.org/10.1007/BF01939906

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  • DOI: https://doi.org/10.1007/BF01939906

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