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Experientia

, Volume 44, Issue 11–12, pp 1007–1010 | Cite as

Vitamins and other metabolites in various sera commonly used for cell culturing

  • H. Baker
  • B. DeAngelis
  • O. Frank
Short Communications

Summary

Many cell culture media use different sera to enhance growth. We assayed vitamins and some related metabolites in different sera and identified the concentration of: thiamin, biotin, folates, riboflavin, pantothenates, nicotinates, vitamins B6, B12, A, E. C, and carotenes and some related metabolites: biopterins, free inositol, free and total choline, total carnitines in chicken, horse, rabbit, goat, pig, calf, newborn calf, fetal calf and human sera. Results indicate that vitamin and metabolite concent of different sera vary. Such variations could produce fluctuant effects on cell culturings if the metabolite content of the serum is not documented.

Key words

Vitamins cell culture 

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Copyright information

© Birkhäuser Verlag Basel 1988

Authors and Affiliations

  • H. Baker
    • 1
  • B. DeAngelis
    • 1
  • O. Frank
    • 1
  1. 1.Department of Preventive Medicine and Community Health, and MedicineNew Jersey Medical SchoolNewarkUSA

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