Skip to main content

Dissociation of digoxin-like immunoreactivity and Na+,K+-ATPase inhibitory activity in rat plasma

Experientia volume 44, pages 992–993 (1988)Cite this article

Summary

We measured endogenous digitalis-like factor (EDF) in rat plasma during acute saline infusion by two different procedures. Na+,K+-ATPase inhibitory activity in the rat plasma significantly increased during saline loading (7.8±2.2 vs 2.5±0.9%, with and without acute saline loading, respectively, p<0.05). On the other hand, the plasma digoxin-like immunoreactivity significantly decreased during acute saline loading (16.9±1.6 vs 32.0±2.8 pg digoxin equivalents/ml. with and without acute saline loading, respectively, p<0.01). These results indicate that the major substances detected by digoxin-like immunoreactivity and direct Na+,K+-ATPase inhibitory activity are completely different, at least in rat plasma.

This is a preview of subscription content, access via your institution.

Access options

Buy single article

Instant access to the full article PDF.

43,34 €

Price includes VAT (Finland)
Tax calculation will be finalised during checkout.

References

  1. Goto, A., Yamada, K., Ishii, M., Yoshioka, M., Ishiguro, T., and Sugimoto, T., Hypertension11 (1988) 645.

    PubMed  Google Scholar 

  2. Goto, A., Yamada, K., Ishii, M., Yoshioka, M., Ishiguro, T., Eguchi, C., and Sugimoto, T., Biochem. biophys. Res. Commun.152 (1988) 322.

    Article  PubMed  Google Scholar 

  3. de Wardener, H. E., Clin. exp. Hypert.A7 (1985) 647.

    Google Scholar 

  4. Poston, L., Clin. Sci.72 (1987) 647.

    PubMed  Google Scholar 

  5. Graves, S. W., and Williams, G. H., A. Rev. Med.38 (1987) 433.

    Article  Google Scholar 

  6. de Wardener, H. E., Millett, J., Holland, S., MacGregor, G. A., and Alaghband-Zadeh, J., Hypertension10 (1987) I51.

    Google Scholar 

  7. Klingmüller, K., Weiler, E., and Kramer, H. J., Klin. Wschr.60 (1982) 1249.

    Article  PubMed  Google Scholar 

  8. Graves, S. W., Hypertension10 (1987) 184.

    Google Scholar 

  9. Scott, P. J., Little, P. J., and Bobik, A., J. Hypert.6 (1988) 205.

    Google Scholar 

  10. Lichtstein, D., and Samuelov, S., Biochem. biophys. Res. Commun.96 (1980) 1518.

    Article  PubMed  Google Scholar 

  11. Haupert, G. T., Hypertension10 (1987) 161.

    Google Scholar 

  12. Favre, H., Siegenthaler, G., Martin, B., Roth, D., Granges, G., and Louis, F., Klin. Wschr.65 (1987) S49.

    Article  Google Scholar 

  13. Gruber, K. A., Whitaker, J. M., and Buckalew, V. M., Nature287 (1980) 743.

    Article  PubMed  Google Scholar 

  14. Yoshioka, M., Yamada, K., Abu-Zeid, M. M., Fujimori, H., Fuke, A., Hirai, K., Goto, A., Ishii, M., and Sugimoto, T., J. Chromat.400 (1987) 133.

    Article  Google Scholar 

  15. Crabos, M., Wainer, I. W., and Cloix, J. F., FEBS Lett.176 (1984) 223.

    Article  PubMed  Google Scholar 

  16. Kelly, R. A., O'Hara, D., Canessa, M. L., Mitch, W. E., and Smith, T. W., J. biol. Chem.260 (1985) 11396.

    PubMed  Google Scholar 

  17. Butler, V. P., and Chen, J. P., Proc. Natl. Acad. Sci. U.S.A.57 (1967) 71.

    PubMed  Google Scholar 

  18. Vasdev, S., Longerich, L., Johnson, E., Brent, D., and Gault, M. H., Res. Commun. chem. Path. Pharmac.49 (1985) 387.

    Google Scholar 

Download references

Author information

Affiliations

  1. Second Department of Internal Medicine, Faculty of Medicine, University of Tokyo, 7-3-1- Hongo, Bunkyo-ku, Tokyo 113, (Japan)

    K. Yamada, A. Goto, M. Ishii & T. Sugimoto

  2. Faculty of Pharmaceutical Science, Setsunan University, Hirakata, (Japan)

    M. Yoshioka

Authors
  1. K. Yamada
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. A. Goto
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. M. Ishii
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. M. Yoshioka
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. T. Sugimoto
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Yamada, K., Goto, A., Ishii, M. et al. Dissociation of digoxin-like immunoreactivity and Na+,K+-ATPase inhibitory activity in rat plasma. Experientia 44, 992–993 (1988). https://doi.org/10.1007/BF01939897

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01939897

Key words

  • Endogenous digitalis-like factor
  • digoxin-like immunoreactivity
  • Na+,K+-ATPase inhibitor