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Experientia

, Volume 46, Issue 6, pp 569–579 | Cite as

Lipid transport pathways in mammalian cells

  • D. R. Voelker
Article

Summary

A major deficit in our understanding of membrane biogenesis in eukaryotes is the definition of mechanisms by which the lipid constituents of cell membranes are transported from their sites of intracellular synthesis to the multiplicity of membranes that constitute a typical cell. A variety of approaches have been used to examine the transport of lipids to different organelles. In many cases the development of new methods has been necessary to study the problem. These methods include cytological examination of cells labeled with fluorescent lipid analogs, improved methods of subcellular fractionation, in situ enzymology that demonstrates lipid translocation by changes in lipid structure, and cell-free reconstitution with isolated organelles. Several general patterns of lipid transport have emerged but there does not appear to be a unifying mechanism by which lipids move among different organelles. Significant evidence now exists for vesicular and metabolic energy-dependent mechanisms as well as mechanisms that are clearly independent of cellular ATP content.

Key words

Lipids membranes transport organelles vesicles 

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Copyright information

© Birkhäuser Verlag 1990

Authors and Affiliations

  • D. R. Voelker
    • 1
  1. 1.Department of MedicineThe National Jewish Center of Immunology and Respiratory MedicineDenverUSA

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