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Estimation of the methylating capacity of the pineal gland. With special reference to indole metabolism

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Summary

In order to obtain more information on the methylating capacity of the pineal gland, a method determining the formation of different 5-methoxyindoles in the pineal gland was developed. The method depends on measuring the incorporation of labelled methyl groups into the various hydroxyindoles present in the pineal gland, after incorporation of pineal tissue with labelled S-adenosyl methionine. Hydroxyindoles were not added to the incubation medium. After incubation thin-layer chromatography was performed with pineal tissue together with the incubation medium; the spots were scraped and counted.

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Authors and Affiliations

  1. Zoological Laboratory, Section Histology and Cell Biology, State University of Utrecht, Utrecht, (The Netherlands)

    M. G. M. Balemans, E. M. Noordegraaf, F. A. M. Bary & M. F. van Berlo

Authors
  1. M. G. M. Balemans
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  2. E. M. Noordegraaf
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  3. F. A. M. Bary
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  4. M. F. van Berlo
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Additional information

The authors wish to express their gratitude to Prof. Dr. J.C. van de Kamer and Dr F.C.G. van de Veerdonk for their stimulating interest in these studies.

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Balemans, M.G.M., Noordegraaf, E.M., Bary, F.A.M. et al. Estimation of the methylating capacity of the pineal gland. With special reference to indole metabolism. Experientia 34, 887–888 (1978). https://doi.org/10.1007/BF01939685

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  • DOI: https://doi.org/10.1007/BF01939685

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