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Large scale isoelectric focusing analysis of the non-histone proteins of the nucleus: Isolation of components with alkaline isoelectric points

Experientia volume 34, page 430 (1978)Cite this article

Summary

After large scale isoelectric focusing of rat liver non-histone protein in polyacrylamide gel, pH range 4–8.6, the only protein material found outside the gradient was present in the cathode solution (20 mM NaOH). This was low mol. wt protein material (approximately 10,000) with an acidic amino acid composition. It bound 5–6 times its own weight of basic ampholine carrier ampholytes to give a complex with a pI of 8.82. This could be dissociated by dialysis against 1 M NaCl.

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References

  1. M. Gronow and G. Griffiths, FEBS Lett.15, 340 (1971).

    PubMed  Google Scholar 

  2. I. L. Cameron and J. R. Jeter, Jr, Acidic Proteins of the Nucleus, Academic Press, London 1974.

    Google Scholar 

  3. R. Barratt and H. J. Gould, Biochim. biophys. Acta294, 165 (1973).

    Google Scholar 

  4. A. J. MacGillivray and D. Rickwood, Eur. J. Biochem.41, 181 (1974).

    PubMed  Google Scholar 

  5. S. Panyim and R. Chalkley, Archs Biochem. Biophys.130, 337 (1969).

    Google Scholar 

  6. K. Weber and M. Osborn, J. biol. Chem.224, 4406 (1969).

    Google Scholar 

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Affiliations

  1. Cancer Research Unit, University of York, Y01 5DD, Heslington, York, (England)

    M. Gronow & T. M. Thackrah

Authors
  1. M. Gronow
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  2. T. M. Thackrah
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Additional information

We are indebted to the Yorkshire Cancer Research Campaign for financial support for this project.

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Gronow, M., Thackrah, T.M. Large scale isoelectric focusing analysis of the non-histone proteins of the nucleus: Isolation of components with alkaline isoelectric points. Experientia 34, 430 (1978). https://doi.org/10.1007/BF01935905

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  • DOI: https://doi.org/10.1007/BF01935905

Keywords

  • Acid Composition
  • Polyacrylamide
  • Amino Acid Composition
  • Isoelectric Point
  • Acidic Amino Acid