Abstract
The avian embryo presents a tremendous challenge for those interested in accessing and manipulating the avian germ line. By far the most successful method of gene transfer is by retrovirus vector. The efficacy of retrovirus vectors has been demonstrated by germ line insertion of replication-competent retroviruses as well as the insertion of replication-defective retrovirus vectors carrying bacterial marker genes. Retroviral vectors have also been shown to be useful for the transfer and expression of genes in somatic cells. Further, germ line transgenesis has been reported in both the chicken and the Japanese quail. In addition, several alternative gene transfer methods are under development. These include transfection of avian sperm, development of germ line chimeras using primordial germ cells and blastodermal cells, and the development of embryonic stem cell lines. Potentially, basic research and the poultry industry will derive substantial benefit from this revolutionary technology.
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Bosselman, R. A., Hsu, R.-Y., Boggs, T., Hu, S., Bruszewski, J., Ou, S., Kozar, L., Martin, F., Green, C., Jacobsen, F., Nicholson, M., Schultz, J. A., Semon, K. M., Rishell, W., and Stewart, R. G., Germline transmission of exogenous genes in chickens. Science243 (1989) 533–535.
Bosselman, R. A., Hsu, R.-Y., Boggs, T., Hu, S., Bruszewski, J., Ou, S., Souza, L., Kozar, L., Martin, F., Nicolson, M., Rishell, W., Schultz, J. A., Semon, K. M., and Stewert, R. G., Replication-defective vectors of reticuloendotheliosis virus transduce exogenous genes into somatic stem cells of the unincubated chicken embryo. J. Virol.63 (1989) 2680–2689.
Brazolot, C. L., Petitte, J. N., Clark M. E., Etches, R. J., and Verrinder-Gibbins, A. M., Introduction of lipofected chicken blastodermal cells into the early chicken embryo. J. cell. Biochem. suppl.15 E (1990) 200.
Briskin, M. J., Hsu, R-Y., Boggs, T., Schultz, J. A., Rishell, W., and Bosselman, R. A., Heritable retroviral transgenes are highly expressed in chickens. Proc. natl. Acad. Sci. USA88 (1991) 1736–1740.
Bulfield, G., The potential for improvement of commercial poultry by genetic engineering techniques, in: Poultry Genetics and Breeding. Eds J. M. Manson and D. Hewitt. Br. Poultry Sci. Symposium16, 1985.
Bumstead, N., Messer, L. I., Freeman, B. M., and Manning, A. C. C., Genetic transformation of chickens using irradiated male gametes. Heredity58 (1987) 25–30.
Chen, H. Y., Garber, E. A., Mills, E., Smith, J., Kopchick, J. J., DiLella, A. G., and Smith, R. G., Vectors, promoters, and expression of genes in chick embryos. J. Reprod. Fert., suppl.41 (1990) 173–182.
Crittenden, L. B., and Salter, D. W., Genetic insertion: current progress and long-term goals. Avian Dis.30 (1986) 43–46.
Crittenden, L. B., Salter, D. W., and Federspiel, M. J., Segregation, viral phenotype, and proviral structure of 23 avian leukosis virus inserts in the germ line of chickens. Theor. appl. Genet.77 (1989) 505–515.
Dickson, C., Eisenman, R., Fan, H., Hunter, E., and Teich, N., Protein biosynthesis and assembly, in: Molecular Biology of Tumor Viruses: RNA Tumor Viruses, 2nd edn, vol. 1, pp. 514–648. Eds R. Weiss, N. Teich, H. Varmus and J. Coffin. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York 1984.
Dickson, C., Eisenman, R., and Fan, H., Protein biosynthesis and assemby, in: Molecular Biology of Tumor Viruses: RNA Tumor Viruses, 2nd edn, vol. 2, pp. 135–145. Eds R. Weiss, N. Teich, H. Varmus and J. Coffin. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York 1985.
Eyal-Giladi, H., and Kochav, S., From cleavage to primitive streak formation: a complementary normal table and a new look at the first stages of the development of the chick. Devl Biol.49 (1976) 321–327.
Freeman, B. M., and Bumstead, N., Transgenic poultry; theory and practice. World's Poultry Sci. J.43 (1987) 180–189.
Freeman, B. M., and Messer, L. I., Genetic manipulation of the domestic fowl. World's Poultry Sci. J.41 (1985) 124–132.
Garber, E. A., Chute, H. T., Condra, J. H., Gotlib, L., Colonno, R. J., Mills, E. O., Hancock, J., Hreniuk, D., and Smith, R. G., Avian cells expressing the murine Mx1 protein are resistant to influenza virus infection. J. cell. Biochem. suppl.15 E (1991) 204.
Greenhouse, J. J., Petropoulos, C. J., Crittenden, L. B., and Hughes, S. H., Helper-independent retrovirus vectors with Rous-associated virus type O long terminal repeats. J. Virol.62 (1988) 4809–4812.
Gruenbaum, Y., Revel, E., Yarus, S., and Fainsod, A., Sperm cells as vectors for the generation of transgenic chickens. J. cell. Biochem. suppl.15 E (1991) 194.
Guido, T. C., Abbott, U. K., and McCarrey, J. R., The interspecific transfer of avian primordial germ cells. J. cell. Biochem. suppl.15 E (1991) 204.
Han, J.-Y., Guise, K. S., ans Shoffner, R. N., Attempts at production of transgenic chickens by manipulated primordial germ cell. J. cell. Biochem. suppl.15 E (1991) 204.
Hughes, S., and Kosik, E., Mutagenesis of the region betweenenv andsrc of the Sr-A strain of Rous sarcoma virus for the purpose of constructing helper-independent vectors. Virology136 (1985) 89–99.
Hughes, S. H., Petropoulos, C. J., Federspiel, M. J., Sutrave, P., Forry-Schaudies, S., and Bradac, J. A., Vectors and genes for improvement of animal strains. J. Reprod. Fert. suppl.41 (1990) 39–49.
Kochav, S., Ginsburg, M., and Eyal-Giladi, H., From cleavage to primitive streak formation: a complementary normal table and a new look at the first stages of the development of the chick. Devl Biol.79 (1980) 296–308.
Lee, M.-R., and Shuman, R. M., Introduction of a bacterial gene into avian embryos by recombinant retrovirus vector. Poultry Sci. suppl.66 (1987) 24.
Lee, M.-R., and Shuman, R. M., Expression of a bacterial marker gene transferred to avian embryos by retrovirus vector. Genome suppl.30 (1988) 448.
Lee, M.-R., and Shuman, R. M., Transgenic quail produced by retrovirus vector infection transmit and express a foreign marker gene. Proc. 4th World Congress on Genetics Applied to Livestock Production, vol. XVI, pp. 107–110. Edinburgh, Scotland 1990.
Nigon, V. M., Chebloune, Y., and Verdier, G., Construction and use of ALV replication devective vectors for germ line infection. Proc. 4th World Congress on Genetics Applied to Livestock Production, vol. XVI, pp. 97–106. Edinburgh, Scotland 1990.
Pandey, K. K., and Patchell, M. R., Genetic transformation in chicken by the use of irradiated male gametes. Molec. gen. Genet.186 (1982) 305–308.
Perry, M. M., A complete culture system for the chick embryo. Nature331 (1988) 70–72.
Perry, M. M., and Sang, H. M., In vitro culture and approaches for DNA transfer in the chick embryo. Proc. 4th World Congress on Genetics Applied to Livestock Production, vol. XVI, pp. 115–118. Edinburgh, Scotland 1990.
Petitte, J. N., Guodong, L., Verrinder-Gibbons, A. M., and Etches, R. J., Production of somatic and germline chimeras in the chicken by transfer of early blastodermal cells. Development108 (1990) 185–189.
Reynaud, G., Transfer de cellules germinales primordiales de dindon à l'embryon de poulet par injection intravasculaire. J. Embryol. exp. Morph.21 (1969) 485–507.
Rosenblum, C. I., and Chen, H. Y., Liposome-mediated gene transfer into chicken embryos in ovo. J. cell. Biochem. suppl.15 E (1991) 208.
Salter, D. W., Balander, R., Bradac, J., Hughes, S., and Crittenden, L. B., Lack of germ-line insertion of avian leukosis proviral DNA in Japanese quail. Poultry Sci. suppl.68 (1989) 129.
Salter, D. W., and Crittenden, L. B., Artificial insertion of a dominant gene for resistance to avian leukosis virus into the germ line of the chicken. Theor. appl. Genet.77 (1989) 457–461.
Salter, D. W., Smith, E. J., Hughes, S. H., Wright, S. E., and Crittenden, L. B., Transgenic chickens: insertion of retroviral genes into the chicken germ line. Virology157 (1987) 235–240.
Salter, D. W., Smith, E. J., Hughes, S. H., Wright, S. E., Fadly, A. M., Witter, R. L., and Crittenden, L. B., Gene insertion into the chicken germ line by retroviruses. Poultry Sci.65 (1986) 1445–1458.
Sang, H. M., and Perry, M., Episomal replication of cloned DNA injected into the fertilised ovum of the hen,Gallus domesticus. Molec. Reprod. Dev.1 (1989) 98–106.
Shimotohno, K., and Temin, H. M., Formation of infectious progeny virus after insertion of herpes simplex thymidine kinase gene into DNA of avian retrovirus. Cell26 (1981) 67–77.
Shoffner, R. N., Perspectives for molecular genetics research and application in poultry. Poultry Sci.65 (1986) 1489–1496.
Shoffner, R. N., Otis, J. S., and Guise, K., Improbability of transvection by irradiated spermatozoa in the chicken. Poultry Sci. suppl.66 (1987) 176.
Shuman, R. M., Primordial germ cell transfer in the chicken,Gallus domesticus. MS Thesis, University of Minnesota, St. Paul 1981.
Shuman, R. M., Leukosis virus envelope sequences produces resistance to Rous sarcoma virus when transferred to cultured cells by retrovirus vector. Poultry Sci. suppl.68 (1989) 133.
Shuman, R. M., Genetic engineering, in: Poultry Breeding and Genetics, pp. 585–598. Ed. R. D. Crawford. Elsevier, Amsterdam 1990.
Shuman, R. M., and Shoffner, R. N., Potential genetic modification in the chicken,Gallus domesticus. Proc. 2nd World's Congress on Genetics Applied to Livestock Production, vol. XI, pp. 157–163. Madrid, Spain, 1982.
Shuman, R. M., and Shoffner, R. N., Gene transfer by avian retroviruses. Poultry Sci.65 (1986) 1437–1444.
Simkiss, K., Vick, L., Luke, G., Page, N., and Sava, D., Infection of primordial germ cells with defective retrovirus and transfer to the developing embryo. Proc. 4th World Congress on Genetics Applied to Livestock Production vol. XVI, pp. 111–114. Edinburgh, Scotland 1990.
Smith, E. J., Endogenous avian leukemia virus, in: Developments in Veterinary Virology, pp. 101–113. Eds Y. Becker and G. F. De Boer. Martinus Nijhoff, Boston 1986.
Soriano, P., and Jaenisch, R., Retroviruses as probes for mammalian development: allocation of cells to the somatic and germ-cell lineages. Cell46 (1986) 19–29.
Souza, L. M., Boone, T. C., Murdock, D., Langley, K., Wypych, J., Fenton, D., Johnson, S., Lai, P. H., Everett, R., Hsu, R.-Y., and Bosselman, R., Applications of recombinant DNA technologies to studies on chicken growth hormone. J. exp. Zool.232 (1984) 465–473.
Temin, H. M., Retrovirus vectors for gene transfer: efficient integration into and expression of exogenous DNA in vertebrate cell genomes, in: Gene Transfer. Ed. R. Kucherlapati. Plenum Press, New York 1986.
Tomita, T., Yamamoto, N., Otsuka, K., Ohta, M., and Hirose, K., Genetic transformation of egg shell colour in chicken by the use of irradiated sperm. Proc. 18th World's Poultry Congress, pp. 515–516. Nagoya, Japan 1988.
Varmus, H., Retroviruses. Science240 (1988) 1427–1435.
Varmus, H. E., and Swanstrom, R., Replication of retroviruses, in: Molecular Biology of Tumor Viruses: RNA Tumor Viruses, 2nd edn, vol. 1, pp. 369–513. Eds R. Weiss, N. Teich, H. Varmus and J. Coffin. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York 1984.
Varmus, H. E., Swanstrom, R., Replication of retroviruses, in: Molecular Biology of Tumor Viruses: RNA Tumor Viruses, 2nd edn, vol. 2, pp. 75–134. Eds R. Weiss, N. Teich, H. Varmus and J. Coffin. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York 1985.
Verrinder-Gibbins, A. M., Brazolot, C. L., Petitte, J. N., Liu, G., and Etches, R. J., Efficient transfection of chicken blastodermal cells and their incorporation into recipient embryos to produce chimeric chicks. Proc. 4th World Congress on Genetics Applied to Livestock Production, vol. XVI, pp. 119–126. Edinburgh, Scotland 1990.
Watanabe, S., and Temin, H. M., Construction of a helper cell line for avian reticuloendotheliosis virus cloning vectors. Molec. cell. Biol.3 (1983) 2241–2249.
Wentworth, B. C., Tsai, H., Hallett, J. H., Gonzales, D. S., and Rajcic-Spasojevic, G., Manipulation of avian primordial germ cells and gonadal differentiation. Poultry Sci.68 (1989) 999–1010.
Yu, S. H., von Rinden, T., Kantoff, P. W., Garber, C., Seiberg, M., Ruther, U., Anderson, W. F., Wagner, E. F., and Gilboa, E., Self-inactivating retroviral vectors designed for transfer of whole genes into mammalian cells. Proc. natl. Acad. Sci. USA83 (1986) 3194–3198.
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Shuman, R.M. Production of transgenic birds. Experientia 47, 897–905 (1991). https://doi.org/10.1007/BF01929880
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DOI: https://doi.org/10.1007/BF01929880