Experientia

, Volume 51, Issue 4, pp 394–397 | Cite as

In vitro development of rat embryos obtained from diabetic mothers

  • E. Menegola
  • M. Prati
  • M. L. Broccia
  • R. Ricolfi
  • E. Giavini
Research Articles
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Abstract

Rat embryos of 9.5 or 10 days of gestation were removed from control or streptozotocin-diabetic mothers and cultured in normal rat serum (180 mg% glucose) or in diabetic serum (600 mg% glucose). The development of control embryos in normal serum was adequate. Embryos from normal mothers cultured in diabetic serum showed signs of developmental retardation. The development of embryos obtained from diabetic mothers was severely impaired, regardless of the gestational age or the culture medium. These results suggest that a diabetic maternal milieu produces irreversible effects in the embryo very early in gestation.

Key words

Diabetes malformations embryo culture 

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Copyright information

© Birkhäuser Verlag Basel 1995

Authors and Affiliations

  • E. Menegola
    • 1
  • M. Prati
    • 1
  • M. L. Broccia
    • 1
  • R. Ricolfi
    • 1
  • E. Giavini
    • 1
  1. 1.Department of BiologyUniversity of MilanMilanItaly

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