, Volume 51, Issue 4, pp 394–397 | Cite as

In vitro development of rat embryos obtained from diabetic mothers

  • E. Menegola
  • M. Prati
  • M. L. Broccia
  • R. Ricolfi
  • E. Giavini
Research Articles


Rat embryos of 9.5 or 10 days of gestation were removed from control or streptozotocin-diabetic mothers and cultured in normal rat serum (180 mg% glucose) or in diabetic serum (600 mg% glucose). The development of control embryos in normal serum was adequate. Embryos from normal mothers cultured in diabetic serum showed signs of developmental retardation. The development of embryos obtained from diabetic mothers was severely impaired, regardless of the gestational age or the culture medium. These results suggest that a diabetic maternal milieu produces irreversible effects in the embryo very early in gestation.

Key words

Diabetes malformations embryo culture 


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  1. 1.
    Eriksson, U., Dahlstrom, E., Larsson, S., and Hellerstrom, C., Diabetes31 (1982) 1.Google Scholar
  2. 2.
    Uriu-Hare, J. J., Stern, J. S., Reaven, G. M., and Keen, C. L., Diabetes34 (1985) 1031.PubMedGoogle Scholar
  3. 3.
    Giavini, E., Broccia, M. L., Prati, M., Roversi, G. D., and Vismara, C., Teratology34 (1986) 81.CrossRefPubMedGoogle Scholar
  4. 4.
    Eriksson, U., Teratology37 (1988) 365.CrossRefPubMedGoogle Scholar
  5. 5.
    Giavini, E., Broccia, M. L., Prati, M., and Roversi, G. D., Biol. Neonate59 (1991) 278.PubMedGoogle Scholar
  6. 6.
    Giavini, E., Airoldi, L., Broccia, M. L., Roversi, G. D., and Prati, M., Biol. Neonate63 (1993) 353.PubMedGoogle Scholar
  7. 7.
    Horton, W. E., and Sadler, T. W., Diabetes32 (1983) 610.PubMedGoogle Scholar
  8. 8.
    Sadler, T. W., Phillips, L. S., Balkan, W., and Goldstein, S., Diabetes35 (1986) 861.PubMedGoogle Scholar
  9. 9.
    Miller, E., Hare, J. W., Cloherty, J. P., Dunn, J. P., Gleason, R. E., Soeldner, J. S., and Kitzmiller, J. L., N. Engl. J. Med.304 (1981) 1331.PubMedGoogle Scholar
  10. 10.
    Fuhrmann, K., Reiher, H., Semmler, K., and Glockner, E., Expl. clin. Endocr.83 (1984) 173.Google Scholar
  11. 11.
    Rosenn, B., Miodovnik, M., Combs, C. A., Khoury, J., Siddiqi, T. A., Obstet. Gynecol.77 (1991) 846.PubMedGoogle Scholar
  12. 12.
    Baker, L., Egler, J. M., Klein, S. H., and Goldman, A. S., Diabetes30 (1981) 955.PubMedGoogle Scholar
  13. 13.
    Eriksson, U., Dahlstrom, E., and Hellerstrom, C., Diabetes32 (1983) 1142.Google Scholar
  14. 14.
    Giavini, E., Broccia, M. L., and Prati, M., Alt. Lab. Anim.19 (1991) 94.Google Scholar
  15. 15.
    Brown, N. A., and Fabro, S., Teratology24 (1981) 65.CrossRefPubMedGoogle Scholar
  16. 16.
    Bradford, M. M., Analyt. Biochem.72 (1976) 248.PubMedGoogle Scholar
  17. 17.
    Pinter, E., Reece, A. E., Ogburn, P. L., Turner, S., Hobbins, J. C., Mahoney, M. J., and Naftolin, F., Am. J. Obstet. Gynecol.159 (1988) 1484.PubMedGoogle Scholar
  18. 18.
    Sadler, T. W., Teratology21 (1980) 349.CrossRefPubMedGoogle Scholar

Copyright information

© Birkhäuser Verlag Basel 1995

Authors and Affiliations

  • E. Menegola
    • 1
  • M. Prati
    • 1
  • M. L. Broccia
    • 1
  • R. Ricolfi
    • 1
  • E. Giavini
    • 1
  1. 1.Department of BiologyUniversity of MilanMilanItaly

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