Abstract
Ecdysteroids in diapause eggs of the silkworm,Bombyx mori, were analyzed using high-performance liquid chromatography (HPLC) combined with radioimmunoassay (RIA). A relatively large amount of an unidentified free ecdysteroid and its phosphoric ester (conjugated form) were detected. These two compounds were isolated by a combination of column chromatography on silicic acid, thin-layer chromatography (TLC), and HPLC using a reverse-phase (RP) column. The purified compounds were identified as 3-epi-22-deoxy-20-hydroxyecdysone (22d20E′) and 3-epi-22-deoxy-20-hydroxyecdysone 2-phosphate (22d20E′2P) by means of mass spectrometry and nuclear magnetic resonance spectroscopy. to our knowledge, this is the first report of 22d20E′ and 22d20E′2P.
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Mamiya, Y., Sonobe, H., Yoshida, K. et al. Occurrence of 3-epi-22-deoxy-20-hydroxyecdysone and its phosphoric ester in diapause eggs of the silkworm,Bombyx mori. Experientia 51, 363–367 (1995). https://doi.org/10.1007/BF01928896
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DOI: https://doi.org/10.1007/BF01928896