Summary
Electron-dense deposits were observed at the base of cilia ofParamecium fixed in glutaraldehyde solution containing 5 mM CaCl2. The deposits were probed by X-ray microanalyzer and it was clearly demonstrated that the deposits consisted of calcium and phosphorus.
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References
Y. Naitoh andH. Kaneko, Science176, 523 (1972).
M. E. J. Holwill andJ. L. McGregor, Nature, Lond.255, 157 (1975).
T. Tsuchiya andK. Takahashi, Annotn. Zool. Japon.45, 63 (1972).
Y. Naitoh andH. Kaneko, J. exp. Biol.58, 657 (1973).
R. Eckert, Science176, 473 (1972).
R. Eckert andY. Naitoh, J. Protozool.19, 237 (1972).
Y. Naitoh, J. gen. Physiol.51, 85 (1968).
T. Tsuchiya andK. Takahashi, in press.
H. Plattner, J. Cell Sci.18, 257 (1975).
H. Plattner andS. Fuchs, Histochemistry45, 23 (1975).
J. L. Oschman, T. A. Hall, P. D. Peters andB. J. Wall, J. Cell Biol.61, 156 (1974).
B. Satir, C. Schooley andP. Satir, Acta protozool.11, 291 (1972).
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The author wishes to express his thanks to Prof.V. Mizuhira of Tokyo Medical and Dental University for his kind guidance and cooperation in performing the X-ray microanalysis. He is also indebted to Prof.H. Sugi for his encouragement and critical reading of the manuscript and Dr.S. Suzuki for his technical advice.
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Tsuchiya, T. Electron microscopy and electron probe analysis of the Ca-binding sites in the cilia ofParamecium caudatum . Experientia 32, 1176–1177 (1976). https://doi.org/10.1007/BF01927611
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DOI: https://doi.org/10.1007/BF01927611