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Mechanism of buffy coat migration inhibition test

Experientia volume 29, pages 340–341 (1973)Cite this article

Résumé

La présence de lymphocytes et la synthèse de la protéine sont nécessaires à la production du MIF (migration inhibition factor). Pour le test de migration des leucocytes du sang périphérique, nous avons pu montrer en utilisant un antigène soluble (PPD) et du sérum de cheval, qu'il n'y a point d'inhibition de migration en l'absence des lymphocytes ou en présence de puromycine. Quand le milieu contient du sérum de sujet testé, l'inhibition n'est que partiellement sensible à la puromycine.

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References

  1. M. Søborg andG. Bendixen, Acta med. scand.181, 247 (1967).

    Article  PubMed  Google Scholar 

  2. K. Federlin, R. Maini, A. Russell andD. Dumonde, J. clin. Path.24, 533 (1971).

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  3. J. Brostoff andI. Roitt, Lancet2, 1269 (1969).

    CAS  Article  PubMed  Google Scholar 

  4. J. Clausen andM. Søborg, Acta med. scand.186, 227 (1969).

    CAS  Article  PubMed  Google Scholar 

  5. R. Rocklin, O. Meyers andJ. David, J. Immun.104, 95 (1970).

    CAS  PubMed  Google Scholar 

  6. M. Lockshin andS. Bombardieri, Immunology20, 641 (1971).

    CAS  PubMed  PubMed Central  Google Scholar 

  7. B. Bloom andB. Bennett, Science153, 80 (1966).

    CAS  Article  PubMed  Google Scholar 

  8. J. David, J. exp. Med.122, 1125 (1965).

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  9. G. Bendixen andM. Søborg, J. Immun.104, 1551 (1970).

    CAS  PubMed  Google Scholar 

  10. R. Maini, personal communication, April 1972.

  11. R. Perper, T. Zee andM. Mickelsen, J. Lab. clin. Med.72, 842 (1968).

    CAS  PubMed  Google Scholar 

  12. J. Waxman andM. Lockshin, Am. Rev. resp. Dis., in press (1972).

  13. S. Rosenberg andJ. David, J. Immun.105, 1447 (1970).

    Google Scholar 

  14. E. Pollock andJ. Vasquez, J. Retic. Soc.10, 205 (1971).

    CAS  Google Scholar 

  15. H. Bartfeld, T. Atoynatan andR. Kelley, Transplantation7, 242 (1969).

    CAS  Article  PubMed  Google Scholar 

  16. P. Glade andK. Hirschhorn, Am. J. Path.60, 483 (1970).

    CAS  PubMed  PubMed Central  Google Scholar 

  17. J. Clausen, Acta allergol.26, 56 (1971).

    CAS  Article  PubMed  Google Scholar 

  18. L. Goldberg, J. Louie andM. Baker, J. Immun.107, 906 (1971).

    CAS  PubMed  Google Scholar 

  19. D. Thor andS. Dray, J. Immun.101, 51 (1968).

    CAS  PubMed  Google Scholar 

  20. J. Zabriskie andR. Falk, Nature, Lond.226, 943 (1970).

    CAS  Article  Google Scholar 

  21. M. Søborg, Acta med. scand.185, 221 (1969).

    Article  PubMed  Google Scholar 

  22. J. Clausen, J. Immun.108, 453 (1972).

    CAS  PubMed  Google Scholar 

  23. B. Nordqvist andH. Rorsman, Acta derm.-vener Stock h50, 435 (1970).

    CAS  Google Scholar 

  24. T. Packalen andJ. Wasserman, Int. Arch. Allerg. appl. Immun.41, 790 (1971).

    CAS  Article  Google Scholar 

  25. E. Heise, S. Han andR. Weiser, J. Immun.101, 1004 (1968).

    CAS  PubMed  Google Scholar 

  26. H. Amos, B. Gurner, R. Olds andR. Coombs, Int. arch. Allerg. appl. Immun.32, 496 (1967).

    CAS  Article  Google Scholar 

  27. M. Lockshin, Proc. Soc. exp. Biol. Med.132, 928 (1969).

    CAS  Article  PubMed  Google Scholar 

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Affiliations

  1. Philip D. Wilson Research Foundation Affiliated with The New York Hospital-Cornell University Medical College, 535 East 70th Street, 10021, New York, New York, USA

    M. D. Lockshin, J. Waxman & M. W. Jenkins

Authors
  1. M. D. Lockshin
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  2. J. Waxman
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  3. M. W. Jenkins
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Additional information

Supported by a grant from the New York Chapter of the Arthritis Foundation.

Post-doctoral Fellow, National Institute of Arthritis and Metabolic disease.

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Lockshin, M.D., Waxman, J. & Jenkins, M.W. Mechanism of buffy coat migration inhibition test. Experientia 29, 340–341 (1973). https://doi.org/10.1007/BF01926517

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  • DOI: https://doi.org/10.1007/BF01926517

Keywords

  • Migration Inhibition Factor
  • Horse Serum
  • Puromycin
  • Nous Avons
  • Soluble Antigen