Summary
Effect of internal colchicine on asymmetrical displacement currents was studied by internally perfusing squid giant axons with a solution containing colchicine. It was found that (1) asymmetrical displacement currents were composed of two parts; colchicine-sensitive and colchicine-resistant; that (2) the colchicine-sensitive part had a definite rising phase while the colchicine-resistant one showed an instantaneous jump, followed by exponential decay; and that (3) the colchicine-sensitive part related to normal Na channels.
Similar content being viewed by others
References
Armstrong, C.M., Bezanilla, F. 1974. Charge movement associated with the opening and closing of the activation gates of the Na channels.J. Gen. Physiol. 63:533–552
Armstrong, C.M., Bezanilla, F. 1977. Inactivation of the sodium channel. II. Gating current experiments.J. Gen. Physiol. 70:567–590
Armstrong, C.M., Gilly, W.F. 1979. Fast and slow steps in the activation of sodium channels.J. Gen. Physiol. 74:691–711
Bezanilla, F., Armstrong, C.M. 1974. Gating currents of the sodium channels: Three ways to block them.Science 183:753–754
Bezanilla, F., Armstrong, C.M. 1975. Kinetic properties and inactivation of the gating currents of sodium channels in squid axon.Philos. Trans. R. Soc. London B 270:449–458
Bezanilla, F., Taylor, R.E. 1978. Temperature effects on gating currents in the squid giant axon.Biophys. J. 23:479–484
Bezanilla, F., Taylor, R.E., Fernández, J.M. 1982. Distribution and kinetics of membrane dielectric polarization 1. Long-term inactivation of gating currents.J. Gen. Physiol. 79:21–40
Gilly, W.F., Armstrong, C.M. 1982. Slowing of sodium channel opening kinetics in squid axon by extracellular zinc.J. Gen. Physiol. 79:935–964
Hodgkin, A.L., Huxley, A.F. 1952. A quantitative description of membrane current and its application to conduction and excitation in nerve.J. Physiol. (London) 117:500–544
Katz, G.M., Schwartz, T.L. 1974. Temporal control of voltageclamped membranes: An examination of principles.J. Membrane Biol. 17:275–291
Keynes, R.D., Rojas, E. 1974. Kinetics and steady-state properties of the charged system controlling sodium conductance in the squid giant axon.J. Physiol. (London) 239:393–434
Keynes, R.D., Rojas, E. 1976. The temporal and steady-state relationship between activation of the sodium conductance and movement of the gating particles in the squid axon.J. Physiol. (London) 255:157–189
Kimura, J.E., Meves, H. 1979. The effect of temperature on the asymmetrical charge movement in squid giant axons.J. Physiol. (London) 289:479–500
Matsumoto, G., Ichikawa, M., Tasaki, A., Murofushi, H., Sakai, H. 1984. Axonal microtubules necessary for generation of sodium current in squid giant axons. I. Pharmacological study on sodium current and Restoration of sodium current by microtubule proteins and 260K protein.J. Membrane Biol. 77:77–91
Meves, H. 1974. The effect of holding potential on the asymmetry currents in squid giant axons.J. Physiol. (London) 243:847–867
Meves, H. 1976. The effect of zinc on the late displacement current in squid giant axons.J. Phusiol. (London) 254:787–801
Meves, H., Vogel, W. 1977a. Inactivation of the asymmetrical displacement current in giant axons ofLoligo forbesi.J. Physiol. (London) 267:377–393
Meves, H., Vogel, W. 1977b. Slow recovery of sodium current and gating current from inactivation.J. Physiol. (London) 267:395–410
Seyama, I., Narahashi, T. 1981. Modulation of sodium channels of squid nerve membranes by grayanotoxin I.J. Pharmacol. Exp. Ther. 219:614–624
Wilson, L. 1970. Properties of colchicine binding protein from chick embryo brain. Interaction with vinca alkaloids and podophyllotoxin.Biochemistry 9:4999–5007
Wilson, L., Bamburg, J.R., Mizel, S.B., Grisham, L.M., Creswell, K.M. 1974. Interaction of drugs with microtubule proteins.Fed. Proc. 33:158–166
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Matsumoto, G., Ichikawa, M. & Tasaki, A. Axonal microtubules necessary for generation of sodium current in squid giant axons: II. Effect of colchicine upon asymmetrical displacement current. J. Membrain Biol. 77, 93–99 (1983). https://doi.org/10.1007/BF01925859
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF01925859