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Experientia

, Volume 30, Issue 7, pp 837–838 | Cite as

Isolement de fragments nucléés et anucléés d'œufs de l'oursinSphaerechinus granularis

Pro Experimentis

Summary

A method for the isolation of nucleate and anucleate fragments ofSphaerechinus granularis unfertilized eggs is described. It is based on centrifugation in a sucrose gradient; its yield is sufficient to allow enzymatic studies to be made.

References

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    E. B. Harvey, Biol. Bull. mar. Biol., Woods Hole71, 101 (1936).Google Scholar
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    S. E. Selvig, P. R. Gross etA. L. Hunter, Devel. Biol.22, 343 (1970).Google Scholar
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    W. G. Lanyon, J. Paul etR. Williamson, Eur. J. Biochem.31, 38 (1972).CrossRefPubMedGoogle Scholar

Copyright information

© Birkhäuser Verlag 1974

Authors and Affiliations

  • J. Aimi
    • 1
  1. 1.Laboratorio di Embriologia molecolareConsiglio Nazionale delle RicercheNapoliItalie

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