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Molecular evidence for increased hematopoietic proliferation in the spleen of the b/b laboratory rat

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Abstract

The splenomegaly and the appearance of a significant number of CFU-E (erythroid colony-forming units) and BFU-E1 (erythroid burst-forming units) in the Belgrade laboratory rat (b/b) spleen prompted us to analyse further the molecular evidence for increased hematopoietic proliferation in the b/b spleen. Messenger RNAs (mRNAs) specific for globins, proteins for iron transport and deposition and the band 3 protein were used in rat erythropoietic tissues as markers for proliferation and erythroid differentiation. In the b/b spleen, all mRNAs analysed display an erythroid-specific pattern of expression. This analysis also revealed an enhanced level of mRNA for ferritin in the +/b spleen, whereas erythrocyte-specific mRNA production was normal.

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Author notes

  1. S. Savković

    Present address: Department of Digestive Diseases and Liver Center (M/C 696), University of Illinois at Chicago, 901 South Wolcott Avenue, 60612, Chicago, Illinois, USA

Authors and Affiliations

  1. Institute of Molecular Genetics and Genetic Engineering, Vojvode Stepe 283, P. O. Box 794, 11001, Beograd, (Yugoslavia)

    S. Savković, S. Pavlović, T. Mitrović, M. Joksimović, J. Marjanović, V. Glišin & Z. Popović

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  1. S. Savković
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  2. S. Pavlović
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  3. T. Mitrović
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  5. J. Marjanović
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  6. V. Glišin
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  7. Z. Popović
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Savković, S., Pavlović, S., Mitrović, T. et al. Molecular evidence for increased hematopoietic proliferation in the spleen of the b/b laboratory rat. Experientia 52, 807–811 (1996). https://doi.org/10.1007/BF01923994

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  • DOI: https://doi.org/10.1007/BF01923994

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