Abstract
We used an enhanced luminescence technique to study the response of rat tissues, such as liver, heart, muscle and blood, to oxidative stress and to determine their antioxidant capacity. As previously found for liver homogenate, the intensity of light emission (E) of tissue homogenates and blood samples, stressed with sodium perborate, is dependent on concentration, and the dose-response curves can be described by the equation E=a·C/exp(b·C). Theb value depends on the antioxidant defence capability of the tissues. In fact, it increases when homogenates are supplemented with an antioxidant, and is correlated with tissue antioxidant capacity, evaluated by two previously set up methods both using the same luminescence technique. Our results indicate that the order of antioxidant capacity of the tissues is liver>blood>heart>muscle. Thea value depends on the systems catalysing the production of radical species. In fact, it is related to the tissue level of hemoproteins, which are known to act as catalysts in radical production from hydroperoxides. The equation proposed to describe the dose-response relation is simple to handle and permits an immediate connection with the two characteristics of the systems analysed which determine their response to the pro-oxidant treatment. However, the equation which best describes the above relation for all the tissues is the following: E=α·C/exp(β·Cδ). The parameter δ assumes values smaller than 1, which seem to depend on relative amounts of tissue hemoproteins and antioxidants. The extension of the analysis to mitochondria shows that they respond to oxidative stress in a way analogous to the tissues, and that the adherence of the dose-response curve to the course predicted from the equation E=a·C/exp(b·C) is again dependent on hemoprotein content.
Similar content being viewed by others
References
Di Meo, S., Venditti, P., Piro, M. C., and De Leo, T., Arch. Physiol. Biochem.103 (1995) 187.
Venditti, P., Di Meo, S., de Martino Rosaroll, P., and De Leo, T., Arch. Physiol. Biochem.103 (1995) 484.
Tappel, A. L., Arch. Biochem. Biophys.44 (1955) 368.
Desai, I. D., and Tappel, A. L., Lipid Res.4 (1963) 204.
Cadenas, E., Boveris, A., and Chance, B., Biochem. J.187 (1980) 131.
Sadrzadeh, S. M. H., Graf, E., Panter, S. S., Hallaway, P. E., and Eaton, J. W., J. biol. Chem.259 (1984) 14354.
Banks, A., Eddie, E., and Smith, J. G. M., Nature (Lond.)190 (1961) 908.
Gutteridge, J. M. C., Richmond R., and Halliwell, B., Biochem. J.184 (1979) 469.
Nishiki, K., Erecinska, M., and Wilson, D. F., Am. J. Physiol.235 (1978) C212.
Winder, W. W., and Holloszy O., Am. J. Physiol.232 (1977) C180.
Azzone, G. V., Colonna, R., and Ziche, B., Methods Enzymol.55 (1979) 46.
Katyare, S. S., Joshi, M. V., Fatterpaker, P., and Sreenivasan, A., Arch. Biochem. Biophys.182 (1977) 155.
Di Meo, S., de Martino Rosaroll, P., and De Leo, T., Cell. Physiol. Biochem.2 (1992) 283.
Gornall, A. G., Bardawill, C. J., and David, M. M., J. biol. Chem.177 (1949) 751.
Aulie, A., and Grav, H. J., Comp. Biochem. Physiol.62A (1979) 335.
Barré, H., Bailly, L., and Rouanet, J. L., Comp. Biochem. Physiol.88B (1987) 519.
Estabrooks, R. W., and Holowinsky, A., J. Biophys. Biochem. Cytol.9 (1961) 19.
Daniels, F., Williams, J. W., Bender, P., Alberty, R. A., and Cornwell, C. D., Experimental Physical Chemistry. McGraw-Hill, New-York 1962.
de Martino Rosaroll, P., Di Maio, V., Valente, M., Di Meo, S., and De Leo, T., J. Endocr. Invest.11 (1988) 559.
Reith, A., Bridiczka, D., Nolte, J., and Staudte, H. W., Expl Cell Res.77 (1973) 155.
Halliwell, B., and Gutteridge, J. M. C., Arch. Biochem. Biophys.280 (1990) 1.
Lew, H., Pyke, S., and Quintanilha, A., FEBS Lett.185 (1985) 262.
Ji, L. L., and Fu, R., J. appl. Physiol.72 (2) (1992) 549.
Lang, J. K., Gohil, K., Packer, L., and Burk, R. F., J. appl. Physiol.63 (6) (1987) 2532.
Gohil, K., Packer, L., de Lumen, B., Brooks, G. A., and Terblanche S. E., J. appl. Physiol.60 (6) (1986) 1986.
Ji, L. L., Med. Sci. Sports Exerc.25 (1993) 225.
Chance, B., Sies, H., and Boveris, A., Physiol. Rev.59 (1979) 527.
Bartoli, G. M., Galeotti, T., and Azzi, A., Biochem. biophys. Acta497 (1977) 622.
Boveris, A., Cadenas, E., and Stoppani, A. O. M., Biochem. J.156 (1976) 435.
Turrens, J. F., and Boveris, A., Biochem. J.191 (1980) 421.
Turrens, J. F., Freeman, B. A., and Crapo, J. D., Arch. Biochem. Biophys.217 (1982) 411.
Sekhar, B. S., Kurup, C. K. R., and Ramasarma, T., J. bioenerg. Biomemb.19 (1987) 397.
Sohal, R. S., Svensson, I., Sohal, B. H., and Brunk, U. T., Mech. ageing Dev.49 (1989) 129.
Sohal, R. S., Svensson, I., and Brunk, U. T., Mech. ageing Dev.53 (1990) 209.
Davies, K. J. A., Quintanilha, A. T., Brooks, G. A., and Packer, L., Biochem. Biophys. Res. Commun.107 (1982) 1198.
Harman, D., Proc. natl Acad. Sci. USA78 (1981) 7124.
Yu, B. P., Physiol. Rev.74 (1994) 139.
Rush, J. D., and Koppenol, W. H., J. biol. Chem.261 (1986) 6730.
Halliwell, B., and Gutteridge, J. M. C., Methods Enzymol.186 (1990) 1.
Gutteridge, J. M. C., Biochem. J.243 (1987) 709.
Stadtman, E. R., A. Rev. Biochem.62 (1993) 797.
Halliwell, B., and Gutteridge, J. M. C., Biochem. J.219 (1984) 1.
Stadtman, E. R., Trends Biochem. Sci. (TIBS)11 (1986) 11.
Rivett, A. J., in: Current Topics in Cellular Regulation, vol. 38, p. 291. Eds B. L. Horecker and E. R. Stadtman. Academic Press, Orlando 1986.
Levine, R. L., J. biol. Chem.258 (1983) 11828.
Gutteridge, J. M. C., FEBS Lett.20 (1986) 291.
Puppo, A., and Halliwell, B., Free Radic. Res. Commun.4 (1988) 415.
Pacifici, R. E., and Davis, K. J. A., Methods Enzymol.186 (1990) 485.
Shigenaga, M. K., Park, J.-W., Cundy, K. C., Gimeno, C. J., and Ames, B. N., Methods Enzymol.186 (1990) 521.
Dillard, C. J., Hu, M.-L., and Tappel, A. L., Free Radic. Biol. Med.10 (1991) 51.
Chen, H., Tappel, A. L., and Boyle, R. C., Free Radic. Biol. Med.14 (1993) 509.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Di Meo, S., Venditti, P. & De Leo, T. Tissue protection against oxidative stress. Experientia 52, 786–794 (1996). https://doi.org/10.1007/BF01923990
Received:
Revised:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF01923990