Experientia

, Volume 52, Issue 8, pp 769–773 | Cite as

Investigations of receptor-mediated phagocytosis by hormone-induced (imprinted)Tetrahymena pyriformis

  • P. Kovacs
  • C. A. Sundermann
  • G. Csaba
Article

Abstract

Receptor-mediated endocytosis byTetrahvmena pyriformis was studied using tetramethylrhodamine isothiocyanate-labeled concanavalin A (TRITC-Con A) with fluorescence and confocal microscopy. In the presence of insulin, or 24 h after insulin pretreatment (hormonal imprinting), the binding and uptake of TRITC-Con A increased when compared to controls, owing to the binding of TRITC-Con A to sugar oligomers of insulin recptors. Mannose inhibited the binding of Con A, thus demonstrating the specificity of binding. Histamine, a phagocytosis-promoting factor in mammals andTetrahymena, and galactose, did not influence the uptake of TRITC-Con A.

Key words

Insulin imprinting Tetrahymena phagocytosis insulin receptor 

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Copyright information

© Birkhäuser Verlag Basel 1996

Authors and Affiliations

  • P. Kovacs
    • 1
  • C. A. Sundermann
    • 2
  • G. Csaba
    • 1
  1. 1.Department of BiologySemmelweis University of MedicineBudapest(Hungary)
  2. 2.Department of Zoology and Wildlife ScienceAuburn UniversityAuburnUSA

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