, Volume 52, Issue 8, pp 769–773 | Cite as

Investigations of receptor-mediated phagocytosis by hormone-induced (imprinted)Tetrahymena pyriformis

  • P. Kovacs
  • C. A. Sundermann
  • G. Csaba


Receptor-mediated endocytosis byTetrahvmena pyriformis was studied using tetramethylrhodamine isothiocyanate-labeled concanavalin A (TRITC-Con A) with fluorescence and confocal microscopy. In the presence of insulin, or 24 h after insulin pretreatment (hormonal imprinting), the binding and uptake of TRITC-Con A increased when compared to controls, owing to the binding of TRITC-Con A to sugar oligomers of insulin recptors. Mannose inhibited the binding of Con A, thus demonstrating the specificity of binding. Histamine, a phagocytosis-promoting factor in mammals andTetrahymena, and galactose, did not influence the uptake of TRITC-Con A.

Key words

Insulin imprinting Tetrahymena phagocytosis insulin receptor 


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  1. 1.
    Csaba, G., Biol. Rev.55 (1980) 47.PubMedGoogle Scholar
  2. 2.
    Csaba, G., Horm. Metab. Res.16 (1984) 329.PubMedGoogle Scholar
  3. 3.
    Kovács, P., and Csaba, G., Comp. Biochem. Physiol.94A (1989) 627.CrossRefGoogle Scholar
  4. 4.
    Kovács, P., and Csaba, G., Cell Biochem. Function.8 (1990) 49.CrossRefGoogle Scholar
  5. 5.
    Christopher, G. K., and Sundermann, C. A., Expl Cell. Res.201 (1992) 477.CrossRefGoogle Scholar
  6. 6.
    Kovács, P., and Csaba, G., Biochem. biophys. Res. Commun.170 (1990) 199.Google Scholar
  7. 7.
    Kovács, P., Csaba, G., Nagao, S., and Nozawa, Y., Microbios59 (1989) 123.PubMedGoogle Scholar
  8. 8.
    Nilsson, J. R., and van Deurs, B., J. Cell Sci.63 (1983) 209.PubMedGoogle Scholar
  9. 9.
    Orias, E., and Rasmussen, L., J. Cell Sci.39 (1979) 343.Google Scholar
  10. 10.
    Nilsson, J. R., in: Biochemistry and Physiology of Protozoa, M. Levandowsky, and S. H. Hutner (eds), pp. 339–379. Academic Press, New York 1979.Google Scholar
  11. 11.
    Ricketts, T. R., J. Protozool.19 (1972) 373.PubMedGoogle Scholar
  12. 12.
    Chiesa, R., Silva, W. I., and Renaud, F. L., J. Eukaryot. Microbiol.40 (1993) 800.PubMedGoogle Scholar
  13. 13.
    Lavin, D. P., Hatzis, C., Srienc, F., and Fredrickson, A. G., Fredrickson, T. Protozool.37 (1990) 157.Google Scholar
  14. 14.
    Rasmussen, L., and Kludt, T. A., Expl Cell. Res.59 (1970) 457.CrossRefGoogle Scholar
  15. 15.
    Csaba, G., and Lantos, T., Cytobiologie7 (1973) 361.Google Scholar
  16. 16.
    Gilboa-Garber, N., and Sharabi, Y., J. Protozool.27 (1980) 109.Google Scholar
  17. 17.
    Csaba, G., and Lantos, T., Experientia32 (1976) 321.PubMedGoogle Scholar
  18. 18.
    Seeman, G. R., J. Protozool.8 (1961) 204.Google Scholar
  19. 19.
    Csaba, G., Int. Rev. Cytol.95 (1985) 327.PubMedGoogle Scholar
  20. 20.
    Kovács, P., Csaba, G., and Lászlo, V., Acta physiol. hung.64 (1984) 19.PubMedGoogle Scholar
  21. 21.
    Katzen, H. M., Soderman, D. D., and Green, B. G., Biochem. biophys Res. Commun.98 (1981) 410.CrossRefPubMedGoogle Scholar
  22. 22.
    Sandra, A., Leon, M. A., and Przybilsky, R. J., Endocrinology105 (1979) 391.PubMedGoogle Scholar
  23. 23.
    Csaba, G., and Kohidai, L., Acta Protozool.25 (1986) 331.Google Scholar
  24. 24.
    Kovács, P., and Csaba, G., Acta physiol. hung.71 (1988) 315.PubMedGoogle Scholar
  25. 25.
    Williams, N. E., Subbaiah, P. V., and Thompson, G. A. Jr, J. biol. Chem.255 (1980) 296.PubMedGoogle Scholar
  26. 26.
    Allen, R. D., J. Cell Biol63 (1974) 904.CrossRefPubMedGoogle Scholar
  27. 27.
    Weidenback, A. L. S., and Thompson, G. A. Jr, J. Protozool.21 (1974) 745.PubMedGoogle Scholar
  28. 28.
    Kushinsky, G., Hille, W., and Shimassek, A., Ach. Pharmakol.266 (1955) 1.Google Scholar
  29. 29.
    Lison, L., and Smoulders, J., C. r. Séanc. Soc. Biol.143 (1949) 575.Google Scholar
  30. 30.
    Christensen, S. T., Cell Biol. Int.11 (1993) 833.CrossRefGoogle Scholar

Copyright information

© Birkhäuser Verlag Basel 1996

Authors and Affiliations

  • P. Kovacs
    • 1
  • C. A. Sundermann
    • 2
  • G. Csaba
    • 1
  1. 1.Department of BiologySemmelweis University of MedicineBudapest(Hungary)
  2. 2.Department of Zoology and Wildlife ScienceAuburn UniversityAuburnUSA

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