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Isolation of thrombin-E and the evolution of enzyme activity from prothrombin

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Résumé

La préthrombine-E a seulement une activité estérasique et, après la perte d'un lien Arg-Ile, elle provoque la formation dlune thrombine protéolytique composée de 2 chaines (A et B) liées dans une maille bisulfide. A la suite d'un procédé de décomposition autolytique, la chaîne B se détache et on obtient la thrombine estérasique formée par 2 chaînes A et B2. On a introduit de nouvelles méthodes pour l'isolement de la thrombine-E. L'antithrombine et le DFP neutralisent effectivement les 3 enzymes.

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Authors and Affiliations

  1. Department of Physiology, Thrombosis Specialized Center of Research, Wayne State University School of Medicine, 540 Canfield Avenue, 48201, Detroit, Michigan, USA

    W. H. Seegers, L. E. McCoy, D. A. Walz, J. Reuterby & T. J. Andary

Authors
  1. W. H. Seegers
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  2. L. E. McCoy
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  3. D. A. Walz
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  4. J. Reuterby
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  5. T. J. Andary
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Additional information

This investigation was supported by research grant No. HL 14142-04 from the National Heart and Lung Institute, National Institutes of Health, U.S. Public Health Service.

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Seegers, W.H., McCoy, L.E., Walz, D.A. et al. Isolation of thrombin-E and the evolution of enzyme activity from prothrombin. Experientia 30, 1130–1132 (1974). https://doi.org/10.1007/BF01923649

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