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Hexosaminidase and alkaline phosphatase activities in articular chondrocytes and relationship to cell culture conditions

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Abstract

Hexosaminidase and alkaline phosphatase activities in rabbit articular chondrocytes have been studied under different cell culture conditions. Chondrocytes were cultured in monolayer primary culture, monolayer subcultured to the fifth passage (in vitro aging) and cultured within a collagen gel; enzymatically released cartilage cells were used as control. Under these conditions, the two enzymes behave quite differently in relationship to alteration of the chondrocyte phenotype in culture. Increased lysosomal hexosaminidase activity could be considered to be a marker of the dedifferentiated phenotype in monolayer subculture; membrane alkaline phosphatase activity could be used as a marker of non-proliferating cells.

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Authors and Affiliations

  1. Laboratoire de Biochimie générale et métabolique, Faculté des Sciences Pharmaceutiques et Biologiques de Paris V, 4 Av. de l'Observatoire, F-75 006, Paris, (France)

    E. Mokonjimobe, D. Robic & R. Bourbouze

  2. Laboratoire de Pharmacologie Cellulaire de l'Ecole Pratique des Hautes Etudes, Centre de Recherches Biomédicales des Cordeliers, 15 rue de l'Ecole de Médecine, F-75006, Paris, (France)

    C. Hecquet & M. Adolphe

Authors
  1. E. Mokonjimobe
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  2. C. Hecquet
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  3. D. Robic
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  4. R. Bourbouze
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  5. M. Adolphe
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Mokonjimobe, E., Hecquet, C., Robic, D. et al. Hexosaminidase and alkaline phosphatase activities in articular chondrocytes and relationship to cell culture conditions. Experientia 48, 396–398 (1992). https://doi.org/10.1007/BF01923440

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  • DOI: https://doi.org/10.1007/BF01923440

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