, Volume 48, Issue 4, pp 374–379 | Cite as

Purification of platelet-derived endothelial cell growth inhibitor and its characterization as transforming growth factor-β type 1

  • St. Magyar-Lehmann
  • P. Böhlen
Research Articles


In 1986, Brown and Clemmons (Proc. natl Acad. Sci. USA83 (1986) 3321) showed that platelets contain a substance, platelet-derived growth inhibitor (PDGI), that inhibits in vitro endothelial cell replication. Although platelets are rich in transforming grwoth factor β (TGF-β), PDGI was considered not to be related to TGF-β, on the basis of its reported properties (extraction from platelets at neutral pH, binding to heparin-Sepharose). However, we purified PDGI to near homogeneity and showed that on the basis of HPLC retention behavior, in vitro growth inhibitory activities with several cell types, receptor binding, and immunoneutralization of growth inhibitory activity with specific anti-TGF-β type 1 antibodies, PDGI is most probably identical with TGF-β type 1.

key words

Platelet-derived growth inhibitor transforming growth factor-β type 1 bovine aortic endothelial cells 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Joseph-Silverstein, J., and Rifkin, D. B., Sem. Thromb. Hemostas.13 (1987) 504.Google Scholar
  2. 2.
    Folkman, J., in: Important Advances in Oncology, p. 42. Eds V. De Vita, S. Hellman, and S. A. Rosenberg. Lippincott, Philadelphia 1985.Google Scholar
  3. 3.
    Folkman, J., Cancer Res.34 (1974) 2109.PubMedGoogle Scholar
  4. 4.
    Esch, F., Baird, A., Ling, N., Ueno, N., Hill, F., Denoroy, L., Klepper, R., Gospodarowicz, D., Böhlen, P., and Guillemin, R., Proc. natl Acad. Sci. USA82 (1985) 6507.PubMedGoogle Scholar
  5. 5.
    Gimenez-Gallego, G., Rodkey, J., Bennett, C., Rios-Candelore, M., DiSalvo, J., and Thomas, K., Science230 (1985) 1385.PubMedGoogle Scholar
  6. 6.
    Ishikawa, F., Miyazono, K., Hellman, U., Drexler, H., Werbstedt, C., Hagiwara, K., Usuki, K., Takaku, F., Risau, W., and Heldin, C. H., Nature338 (1989) 557.PubMedGoogle Scholar
  7. 7.
    Leung, D. W., Cachianes, G., Kuang, W. J., Goeddel, D., and Ferrara, N., Science246 (1989) 1306.PubMedGoogle Scholar
  8. 8.
    Keck, P. J., Hauser, S. D., Krivi, G., Sanzo, K., Warren, T., Feder, J., and Conolly, D. T., Science246 (1989) 1309.PubMedGoogle Scholar
  9. 9.
    Folkman, J., and Klagsbrun, M., Science235 (1987) 442.PubMedGoogle Scholar
  10. 10.
    Frater-Schröder, M., Müller, G., Birchmeier, W., and Böhlen, P., Biochem. biophys. Res. Commun.137 (1986) 295.PubMedGoogle Scholar
  11. 11.
    Baird, A., and Durkin, T., Biochem. biophys. Res. Commun.138 (1986) 476.PubMedGoogle Scholar
  12. 12.
    Heimark, R., Twardzik, D., and Schwartz, S., Science233 (1986) 1078.PubMedGoogle Scholar
  13. 13.
    Sato, N., Goto, K., Haranaka, N., Satomi, H., Nariuchi, Y., Mano-Hirano, Y., and Sawasaki, Y., J. natl Cancer Inst.76 (1986) 1113.PubMedGoogle Scholar
  14. 14.
    Frater-Schröder, M., Risau, W., Hallmann, R., Gautschi, P., and Böhlen, P., Proc. natl Acad. Sci. USA84 (1987) 5277.PubMedGoogle Scholar
  15. 15.
    Stolpen, A., Guinan, W., Fiers, W., and Pober, J., Am. J. Path.123 (1986) 16.PubMedGoogle Scholar
  16. 16.
    Gaffney, E. V., and Tsai, S. C., Cancer Res.46 (1986) 3834.PubMedGoogle Scholar
  17. 17.
    Tsai, S. C., and Gaffney, E. V., Cancer Res.46 (1986) 1471.PubMedGoogle Scholar
  18. 18.
    Heyns, A., Eldor, I., Vlodavsky, N., Kaiser, N., Fridman, R., and Panet, A., Exp. Cell Res.161 (1985) 297.PubMedGoogle Scholar
  19. 19.
    Böhlen, P., Frater-Schröder, M., Michel, T., and Jiang, Z. P., in: Angiogenesis, p. 119. Eds D. Rifkin and M. Klagsbrun. Current Communications in Molecular Biology, Cold Spring Harbor Laboratory, Cold Spring Harbor 1987.Google Scholar
  20. 20.
    Wright, T. C. Jr, Johnstone, T. V., Castellot, J. J., and Karnovsky, M. J., J. cell. Physiol.125 (1985) 499.PubMedGoogle Scholar
  21. 21.
    Willems, C., Astaldi, G. C. B., DeGroot, P. G., Janssen, M. C., Gonsalvez, M. D., Zeijlemakerr, W. P., Van Mourik, J. A., and Van Aken, W. G., Exp. Cell Res.139 (1982) 191.PubMedGoogle Scholar
  22. 22.
    Brown, M. T., and Clemmons, D. R., Proc. natl Acad. Sci. USA83 (1986) 3321.PubMedGoogle Scholar
  23. 23.
    Brem, H., and Folkman, J., J. exp. Med.141 (1975) 427.PubMedGoogle Scholar
  24. 24.
    Lutty, G. A., Thompson, D. C., Gallup, J. Y., Mello, R. J., Patz, A., and Fenselau, A., Invest. Ophthalm. vis. Sci.23 (1983) 52.Google Scholar
  25. 25.
    Seyedin, S. M., Thomas, T. C., Thompson, A. Y., Rosen, D. M., and Piez, K. A., Proc. natl Acad. Sci. USA82 (1985) 2267.PubMedGoogle Scholar
  26. 26.
    Williams, G. A., Eisenstein, R., Schumacher, B., Hsiao, K. C., and Grant, D., Am. J. Ophthalm.97 (1984) 366.Google Scholar
  27. 27.
    Takigawa, M., Shirai, E., Enomoto, M., Hiraki, Y., Fukuya, M., Suzuki, F., Suiio, T., and Yugari, Y., Cell Biol. int. Reports9 (1985) 619.Google Scholar
  28. 28.
    Schumacher, B., Grant, D., and Eisenstein, R., Arteriosclerosis5 (1985) 110.PubMedGoogle Scholar
  29. 29.
    Homandberg, G., Williams, J., Grant, D., Schumacher, B., and Eisenstein, R., Am. J. Physiol.120 (1985) 327.Google Scholar
  30. 30.
    Glaser, B. M., Campochiaro, P. A., Davis, J. L. Jr, and Sato, M., Archs Ophthalm.103 (1985) 1870.Google Scholar
  31. 31.
    Lutty, G. A., Mello, R. J., Chandler, C., Fait, C., Bennett, A., and Patz, A., J. cell. Sci.76 (1985) 53.PubMedGoogle Scholar
  32. 32.
    Lee, A., and Langer, R., Science221 (1983) 1185.PubMedGoogle Scholar
  33. 33.
    Eisenstein, R., Goren, S. B., Schumacher, B., and Choromokos, E., Am. J. Ophthalm.88 (1979) 1005.Google Scholar
  34. 34.
    Rastinejad, F., Polverini, P. J., and Bouck, N. P., Cell56 (1989) 345.PubMedGoogle Scholar
  35. 35.
    Assoian, R. K., Komoriya, A., Meyers, C. A., Miller, D., and Sporn, M. B., J. biol. Chem.258 (1983) 7155.PubMedGoogle Scholar
  36. 36.
    Bradford, M. M., Analyt. Biochem.72 (1976) 248.PubMedGoogle Scholar
  37. 37.
    Gospodarowicz, D., Cheng, J., Lui, G. M., Baird, A., and Böhlen, P., Proc. natl Acad. Sci. USA81 (1984) 6963.PubMedGoogle Scholar
  38. 38.
    Böhlen, P., Esch, F., Baird, A., and Gospodarowicz, D., EMBO J.4 (1985) 1951.PubMedGoogle Scholar
  39. 39.
    Böhlen, P., Baird, A., Esch, F., Ling, N., and Gospodarowicz, D., Proc. natl Acad. Sci. USA81 (1984) 5364.PubMedGoogle Scholar
  40. 40.
    Sharma, C. P., and Gehring, H., Biochem. biophys. Res. Commun.39 (1986) 1243.Google Scholar
  41. 41.
    Koyama, H., Goodpasture, C., Miller, M. M., Teplitz, R. L., and Riggs, A. D., In Vitro14 (1978) 239.PubMedGoogle Scholar
  42. 42.
    Danielpour, D., Dart, L. L., Flanders, K. C., Roberts, A. B., and Sporn, M. B., J. cell. Physiol.138 (1989) 79.PubMedGoogle Scholar
  43. 43.
    Frolik, C. A., Wakefield, L. M., Smith, D. M., and Sporn, M. B., J. biol. Chem.259 (1984) 10995.PubMedGoogle Scholar
  44. 44.
    Sporn, M. B., Roberts, A. B., Wakefield, L. M., and deCrombrugghe, B., J. Cell Biol.105 (1987) 1039.PubMedGoogle Scholar
  45. 45.
    Anzano, M. A., Roberts, A. B., and Sporn, M. B., J. cell. Physiol.126 (1986) 312.PubMedGoogle Scholar

Copyright information

© Birkhäuser Verlag 1992

Authors and Affiliations

  • St. Magyar-Lehmann
    • 1
  • P. Böhlen
    • 1
  1. 1.Institute of BiochemistryUniversity of ZürichZürich(Switzerland)

Personalised recommendations