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In situ hybridization of rDNA on chromosomes of the honeybee,Apis mellifera L.


DNA probes containing the repeated rDNA region ofDrosophila melanogaster (coding for e.g. 28S and 18S rRNA) hybridized in situ to distinct regions of two heterologous mitotic chromosomes of the honeybee, identifying the nucleolus organizing regions (NORs). The method allows a rapid establishment of a physical map ofApis mellifera using other DNA probes ofDrosophila. This is the first report on well-defined chromosomal markers in the honeybee.

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Beye, M., Moritz, R.F.A. In situ hybridization of rDNA on chromosomes of the honeybee,Apis mellifera L.. Experientia 49, 337–338 (1993).

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Key words

  • Apis mellifera
  • fluorescence in situ hybridization
  • rDNA
  • chromosome