Experientia

, Volume 52, Issue 2, pp 159–166 | Cite as

2-Carboxyethylgermanium sesquioxide, a synthetic organogermanium compound, as an inducer of contrasuppressor T cells

  • K. Ikemoto
  • M. Kobayashi
  • T. Fukumoto
  • M. Morimatsu
  • R. B. Pollard
  • F. Suzuki
Research Articles

Abstract

2-Carboxyethylgermanium sesquioxide (Ge-132), a synthesized organogermanium compound with immunomodulaing activities, was shown to be an inducer of anti-suppressor T cells in normal mice. The suppressor cell activity of T6S cells, a clone of burn-induced CD8+ IL-4-producing suppressor T cells, was clearly inhibited when a mixed lymphocyte-tumor cell reaction of the clone was conducted with splenic mononuclear cells from mice treated orally with a 100 mg/kg dose of Ge-132. The activity of anti-suppressor cells was demonstrated in spleens of mice 2 days after treatment with Ge-132 and reached its peak on day 3. The anti-suppressor cells induced by the compound were of a contrasuppressor T cell-linage, because they were characterized as CD4+ CD28+ TCRα/β+Vicia villosa lectin-adherent T cells. These cells produced IFN-γ but did not produce IL-2, IL-4, IL-6 or IL-10 in their culture fluids. CD4+ anti-suppressor T cells induced by Ge-132 may be different from other subsets of CD4+ T cells because Th1 and Th2 cells generated in our laboratory did not adhere toVicia villosa lectin-coated petri dishes, and each produced specific cytokines. Th1 cells produced IFN-γ and IL-2 while Th2 cells produce IL-4 and IL-10 in vitro. These results suggest that Ge-132 may be useful as an inducer of contrasuppressor T cells in immunocompromised individuals bearing suppressor T cells. To eliminate suppressor T cells from immunocompromised hosts may result in improved resistance from various opportunistic infections.

Key words

Organogermanium compound contrasuppressor T cells suppressor T cells 

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Copyright information

© Birkhäuser Verlag Basel 1996

Authors and Affiliations

  • K. Ikemoto
    • 1
  • M. Kobayashi
    • 3
  • T. Fukumoto
    • 2
  • M. Morimatsu
    • 1
  • R. B. Pollard
    • 3
  • F. Suzuki
    • 3
  1. 1.Department of NeurologyYamaguchi University of Medical SchoolYamaguchi(Japan)
  2. 2.Department of AnatomyYamaguchi University of Medical SchoolYamaguchi(Japan)
  3. 3.Department of Internal MedicineThe University of Texas Medical Branch and Shriners Burns InstituteGalvestonUSA)

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