Abstract
A cytosolic 23kDa protein was initially puified from bovine brain and shown to bind phosphatidylethanolamine. Later, it was also characterized in rat and human brain, and it is now known to be widespread, having been found in numerous tisues in several species. Here, we report the high level of mRNA and phosphatidyl ethanolamine binding protein expression in rat testis and to a lesser extent mouse testis. In human testis, although it was not detectable by Northern blot analysis, the mRNA was shown be present when PCR amplificatin was performed. Immunohistochemical experiments revealed that the testicular phosphatidylethanolamine binding protein (tPBP) is principally expressed in the elongated spermatids of both rat and mouse testis. This finding, and the association of tPBP with cellular membranes, suggest its possible implication in membrane remodelling during spermatid maturation.
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Seddiqi, N., Segretain, N.D., Bucquoy, S. et al. Characterization and localization of the rat, mouse and human testicular phosphatidylethanolamine binding protein. Experientia 52, 101–110 (1996). https://doi.org/10.1007/BF01923352
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DOI: https://doi.org/10.1007/BF01923352