, Volume 31, Issue 12, pp 1418–1420 | Cite as

Use of a tissue culture medium for in vitro studies on the ion transport capacity of amphibian epithelia

  • M. G. Emílio
  • M. Balls
  • H. Menano
Specialia Physiologica


The sodium transport capacity across frog skin and toad bladder in vitro is enhanced when a tissue culture medium is used to mount the preparations, instead of the classic Ringer solution. The response to antidiuretic hormone used 24 h after isolating the tissues is also higher in preparations incubated in the same culture medium.


Sodium Tissue Culture Antidiuretic Hormone Transport Capacity Ringer Solution 
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Copyright information

© Birkhäuser Verlag 1975

Authors and Affiliations

  • M. G. Emílio
    • 1
    • 2
  • M. Balls
    • 1
    • 2
  • H. Menano
    • 1
    • 2
  1. 1.Gulbenkian Institute of ScienceCentro de BiologiaOeiras(Portugal)
  2. 2.School of Biological SciencesUniversity of East AngliaNorwich(England)

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