Use of a tissue culture medium for in vitro studies on the ion transport capacity of amphibian epithelia
The sodium transport capacity across frog skin and toad bladder in vitro is enhanced when a tissue culture medium is used to mount the preparations, instead of the classic Ringer solution. The response to antidiuretic hormone used 24 h after isolating the tissues is also higher in preparations incubated in the same culture medium.
KeywordsSodium Tissue Culture Antidiuretic Hormone Transport Capacity Ringer Solution
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