Advertisement

Experientia

, Volume 51, Issue 8, pp 804–808 | Cite as

Sr2+ can become incorporated into an agonist-sensitive, cytoplasmic Ca2+ store in a cell line derived from the equine sweat gland epithelium

  • W. H. Ko
  • J. D. Pediani
  • D. L. Bovell
  • S. M. Wilson
Research Articles

Abstract

We have explored the properties of a Ca2+-dependent cell-signalling pathway that becomes active when cultured equine sweat gland cells are stimulated with ATP. The ATP-regulated, Ca2+-influx pathway allowed Sr2+ to enter the cytoplasm but permitted only a minimal influx of Ba2+. Experiments in which cells were repeatedly stimulated with ATP suggested that Sr2+, but not Ba2+, could become incorporated into the agonist-sensitive, cytoplasmic Ca2+ store. Further evidence for this was provided by experiments using ionomycin, a Ca2+ ionophore which has no affinity for Sr2+.

Key words

Ca2+ mobilisation purinergic receptors nucleotide receptors sweat gland epithelial cells ionomycin Fura-2 horse 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Dubyak, G. R., and El-Moatassim, C., Am. J. Physiol.265 (1993) C577.PubMedGoogle Scholar
  2. 2.
    Burnstock, G., Neurochem. Int.17 (1990) 251.Google Scholar
  3. 3.
    Ko, W. H., O'Dowd, J. J. M., Pediani, J. D., Elder, H. Y., Bovell, D. L., Jenkinson, D. M., and Wilson, S. M., J. expl Biol190 (1994) 239.Google Scholar
  4. 4.
    Berridge, M. J., Nature361 (1993) 315.PubMedGoogle Scholar
  5. 5.
    Kwan, C. Y., and Putney, J. W., J. biol. Chem.265 (1990) 678.PubMedGoogle Scholar
  6. 6.
    Leung, A. Y. H., Tai, H. L., and Wong, P. Y. D., Am. J. Physiol.264 (1993) C1388.PubMedGoogle Scholar
  7. 7.
    Schilling, W. P., Rejan, L., and Strobl-Jager, E., J. biol. Chem.264 (1989) 12838.PubMedGoogle Scholar
  8. 8.
    Ko, W. H., Wilson, S. M., and Elder, H. Y., J. Physiol., Lond.475 (1994) 101P.Google Scholar
  9. 9.
    Wilson, S. M., Pediani, J. D., Ko, W. H., Bovell, D. L., Kitson, S., Montgomery, I., Brown, U. M. D., Smith, G. L., Elder, H. Y., and Jenkinson, D. McE., J. expl. Biol.183 (1993) 179.Google Scholar
  10. 10.
    Lui, C., and Hermann, T. E., J. biol. Chem.253 (1978) 5892.PubMedGoogle Scholar
  11. 11.
    Grynkiewicz, G., Poenie, M., and Tsien, R. Y., J. biol. Chem.265 (1985) 3440.Google Scholar
  12. 12.
    Merritt, J. E., and Hallam, T. J., J. biol. Chem.263 (1988) 6161.PubMedGoogle Scholar
  13. 13.
    Yamaguchi, D. T., Green, J., Kleeman, C. R., and Muallem, S., J. biol. Chem.264 (1989) 197.PubMedGoogle Scholar
  14. 14.
    Gill, D. L., Grollman, E. F., and Kohn, L. D., J. biol. Chem.256 (1981) 184.PubMedGoogle Scholar
  15. 15.
    Gill, D. L., and Chueh, S. H., J. biol. Chem.260, (1985) 9289.PubMedGoogle Scholar
  16. 16.
    Somlyo, A. P., Somlyo, A. V., Devine, C. E., Peters, P. D., and Hall, T. A., J. Cell Sci.61 (1974) 723.Google Scholar
  17. 17.
    Morgan, A. J., and Jacob, R., J. Physiol., Lond.475 (1994) 65P.Google Scholar
  18. 18.
    Stuenkel, E. L., and Ernst, S. A., Am. J. Physiol.258 (1990) C289.PubMedGoogle Scholar
  19. 19.
    Bijman, J., and Quinton, P. M., Am. J. Physiol.247 (1984) C10.PubMedGoogle Scholar
  20. 20.
    Bijman, J., and Quinton, P. M., Am. J. Physiol.246 (1984) R349.PubMedGoogle Scholar
  21. 21.
    Wilson, S. M., Ko, W. H., Pediani, J. D., Rakhit, S., Nichol, J. A., and Bovell, D. L., Comp. Biochem. Physiol (1995) in press.Google Scholar
  22. 22.
    Petersen, O. H., J. Physiol., Lond.448 (1992) 1.Google Scholar
  23. 23.
    Thastrup, O., Cullen, P. J., Drobak, B. K., Hanley, M. R., and Dawson, A. P., Proc. natl Acad. Sci. USA87 (1990) 2466.PubMedGoogle Scholar
  24. 24.
    Takemura, H., Hughes, A. R., Thastrup, O., and Putney, J. W., J. biol. Chem.264 (1989) 12266.PubMedGoogle Scholar
  25. 25.
    Kwan, C. Y. Takemura, H., Obie, J. F., Thapstrup, O., and Putney, J. W., Am. J. Physiol.258 (1990) C1006.PubMedGoogle Scholar

Copyright information

© Birkhäuser Verlag 1995

Authors and Affiliations

  • W. H. Ko
    • 3
  • J. D. Pediani
    • 1
  • D. L. Bovell
    • 2
  • S. M. Wilson
    • 1
  1. 1.Division of Neuroscience and Biomedical Systems, Institute of Biomedical and Life SciencesUniversity of GlasgowGlasgow(Scotland, UK)
  2. 2.Department of Biological SciencesGlasgow Caledonian UniversityGlasgow(Scotland, UK)
  3. 3.Department of PhysiologyThe Chinese University of Hong Kong(Hong Kong)

Personalised recommendations